PRDM14 suppresses expression of differentiation marker genes in human embryonic stem cells

被引：91

作者：

Tsuneyoshi, Norihiro ^{[1
,2
]}

Sumi, Tomoyuki ^{[1
]}

Onda, Hiroaki ^{[3
]}

Nojima, Hiroshi ^{[3
,4
]}

Nakatsuji, Norio ^{[2
,5
]}

Suemori, Hirofumi ^{[1
]}

机构：

[1] Kyoto Univ, Inst Frontier Med Sci, Stem Cell Res Ctr, Lab Embryon Stem Cell Res,Sakyo Ku, Kyoto 6068507, Japan

[2] Kyoto Univ, Inst Frontier Med Sci, Dept Dev & Differentiat, Sakyo Ku, Kyoto 6068507, Japan

[3] Osaka Univ, Res Inst Microbial Dis, Dept Mol Genet, Suita, Osaka 5650871, Japan

[4] Osaka Univ, Res Inst Microbial Dis, DNA Chip Dev Ctr Infect Dis, Suita, Osaka 5650871, Japan

[5] Kyoto Univ, Inst Integragted Cell Mat Sci, Sakyo Ku, Kyoto 6068501, Japan

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2008年 / 367卷 / 04期

基金：

日本学术振兴会;

关键词：

human ES cells; self-renewal; pluripotency; PRDM14; PFM11; PR domain; SET domain;

D O I：

10.1016/j.bbrc.2007.12.189

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

PRDM14 was identified by microarray analysis and was expressed in specifically undifferentiated human ES cells. PRDM14 protein is thought to regulate gene transcription in human ES cells, as it contains a PR domain, a subtype of the SET domain which catalyzes historic methylation. To analyze the function of PRDM14, we performed knock-down and forced expression of PRDM14 in human ES cells. Knock-down of PRDM14 by siRNA induced expression of early differentiation marker genes. Forced expression of PRDM14 suppressed expression of differentiation marker genes in the embryoid body. These results suggest that PRDM14 is involved in the maintenance of the self-renewal of human ES cells by suppression of gene expression. (c) 2008 Elsevier Inc. All rights reserved.

引用

页码：899 / 905

页数：7

共 36 条

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