Molecular cloning and characterization of stanniocalcin-related protein

被引：50

作者：

DiMattia, GE ^{[1
]}

Varghese, R

Wagner, GF

机构：

[1] Univ Western Ontario, Dept Oncol, London, ON, Canada

[2] Univ Western Ontario, Dept Biochem, London, ON, Canada

[3] Univ Western Ontario, Dept Physiol, London, ON, Canada

[4] London Reg Canc Ctr, London, ON N6A 4L6, Canada

来源：

MOLECULAR AND CELLULAR ENDOCRINOLOGY | 1998年 / 146卷 / 1-2期

基金：

英国医学研究理事会;

关键词：

stanniocalcin; stanniocalcin-related protein; hormone; molecular cloning; gene expression;

D O I：

10.1016/S0303-7207(98)00163-4

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Stanniocalcin (STC is a glycoprotein hormone first discovered in fish and recently identified in humans and mice. In this report we have described the cloning of an STC-like cDNA, designated here as STC related protein (STCrP). Human STCrP (hSTCrP) cDNAs were isolated as expressed sequence tags (ESTs) and predicted a polypeptide of 302 amino acids, with 58% homology to human STC (hSTC). Ten of the eleven 1/2 cysteine residues that in STC allow for dimerization of monomeric subunits were conserved in hSTCrP. By Northern analysis, three hSTCrP mRNAs were detected and were most abundant in pancreas, spleen and kidney as well as a variety of different transformed cell lines. The high degree of sequence homology suggests that STC and STCrP may have been derived from a common ancestral gene. (C) 1998 Elsevier Science Ireland Ltd. All rights reserved.

引用

收藏

页码：137 / 140

页数：4

相关论文

共 13 条

[1] A NOVEL HUMAN CDNA HIGHLY HOMOLOGOUS TO THE FISH HORMONE STANNIOCALCIN [J].

CHANG, ACM ;

JANOSI, J ;

HULSBEEK, M ;

DEJONG, D ;

JEFFREY, KJ ;

NOBLE, JR ;

REDDEL, RR .

MOLECULAR AND CELLULAR ENDOCRINOLOGY, 1995, 112 (02) :241-247

[2]

CHOMCZYNSKI P, 1987, ANAL BIOCHEM, V162, P156, DOI 10.1016/0003-2697(87)90021-2

[3] STANNIOCALCIN STIMULATES PHOSPHATE REABSORPTION BY FLOUNDER RENAL PROXIMAL TUBULE IN PRIMARY CULTURE [J].

LU, MQ ;

WAGNER, GF ;

RENFRO, JL .

AMERICAN JOURNAL OF PHYSIOLOGY-REGULATORY INTEGRATIVE AND COMPARATIVE PHYSIOLOGY, 1994, 267 (05) :R1356-R1362

[4] Stanniocalcin: a novel protein regulating calcium and phosphate transport across mammalian intestine [J].

Madsen, KL ;

Tavernini, MM ;

Yachimec, C ;

Mendrick, DL ;

Alfonso, PJ ;

Buergin, M ;

Olsen, HS ;

Antonaccio, MJ ;

Thomson, ABR ;

Fedorak, RN .

AMERICAN JOURNAL OF PHYSIOLOGY-GASTROINTESTINAL AND LIVER PHYSIOLOGY, 1998, 274 (01) :G96-G102

[5] Human stanniocalcin: A possible hormonal regulator of mineral metabolism [J].

Olsen, HS ;

Cepeda, MA ;

Zhang, QQ ;

Rosen, CA ;

Vozzolo, BL ;

Wagner, GF .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1996, 93 (05) :1792-1796

[6] Defining the roles of parathyroid hormone-related protein in normal physiology [J].

Philbrick, WM ;

Wysolmerski, JJ ;

Galbraith, S ;

Holt, E ;

Orloff, JJ ;

Yang, KH ;

Vasavada, RC ;

Weir, EC ;

Broadus, AE ;

Stewart, AF .

PHYSIOLOGICAL REVIEWS, 1996, 76 (01) :127-173

[7] Comparative analysis of mammalian stanniocalcin genes [J].

Varghese, R ;

Wong, CKC ;

Deol, H ;

Wagner, GF ;

DiMattia, GE .

ENDOCRINOLOGY, 1998, 139 (11) :4714-4725

[8] A NEW METHOD FOR PREDICTING SIGNAL SEQUENCE CLEAVAGE SITES [J].

VONHEIJNE, G .

NUCLEIC ACIDS RESEARCH, 1986, 14 (11) :4683-4690

[9] IMMUNOLOGICAL AND BIOLOGICAL EVIDENCE FOR A STANNIOCALCIN-LIKE HORMONE IN HUMAN KIDNEY [J].

WAGNER, GF ;

GUIRAUDON, CC ;

MILLIKEN, C ;

COPP, DH .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1995, 92 (06) :1871-1875

[10] Human stanniocalcin inhibits renal phosphate excretion in the rat [J].

Wagner, GF ;

Vozzolo, BL ;

Jaworski, E ;

Haddad, M ;

Kline, RL ;

Olsen, HS ;

Rosen, CA ;

Davidson, MB ;

Renfro, JL .

JOURNAL OF BONE AND MINERAL RESEARCH, 1997, 12 (02) :165-171