DNA in situ hybridization for the rapid diagnosis of massive necrotizing avian adenovirus hepatitis and pancreatitis in chicks

被引：11

作者：

Goodwin, MA ^{[1
]}

Latimer, KS ^{[1
]}

Resurreccion, RS ^{[1
]}

Miller, PG ^{[1
]}

Campagnoli, RP ^{[1
]}

机构：

[1] UNIV GEORGIA,COLL VET MED,DEPT VET PATHOL,ATHENS,GA 30602

来源：

AVIAN DISEASES | 1996年 / 40卷 / 04期

关键词：

DNA in situ hybridization; inclusion body; avian; adenovirus; pancreatitis; hepatitis; chicks;

D O I：

10.2307/1592305

中图分类号：

S85 [动物医学（兽医学）];

学科分类号：

0906 ;

摘要：

The present study describes the use of DNA in situ hybridization for the rapid diagnosis of massive necrotizing adenovirus hepatitis and pancreatitis in broiler chicks. A light microscope and DNA probes were used to identify avian adenovirus in replicate sections of formalin-fixed paraffin-embedded liver and pancreas from field and experimental chicks. Avian adenovirus infection was confirmed by transmission electron microscopy and virus isolation.

引用

页码：828 / 831

页数：4

共 14 条

[1]

BARR DA, 1988, POULTRY DIS, V112, P323

[2]

BULOW VV, 1986, CURRENT TOPICS VET M, V37, P203

[3] A PRIMARY EPIDEMIC OF INCLUSION BODY HEPATITIS IN BROILERS [J].

CHRISTENSEN, NH ;

SAIFUDDIN, M .

AVIAN DISEASES, 1989, 33 (04) :622-630

[4]

DOANE FW, 1987, ELECTRON MICROS, P69

[5] MOLECULAR CHARACTERIZATION OF HIGHLY VIRULENT FOWL ADENOVIRUSES ASSOCIATED WITH OUTBREAKS OF INCLUSION BODY HEPATITIS [J].

ERNY, KM ;

BARR, DA ;

FAHEY, KJ .

AVIAN PATHOLOGY, 1991, 20 (04) :597-606

[6] DIAGNOSIS OF POLYOMAVIRUS INFECTION IN SEEDCRACKERS (PYRENESTES SP) AND BLUE BILLS (SPERMOPHAGA-HAEMATINA) USING DNA IN-SITU HYBRIDIZATION [J].

GARCIA, A ;

LATIMER, KS ;

NIAGRO, FD ;

NORTON, TM ;

CAMPAGNOLI, RP ;

HARMON, BG ;

HOWERTH, EW ;

RITCHIE, BW .

AVIAN PATHOLOGY, 1994, 23 (03) :525-537

[7]

Garcia AnaPatricia, 1993, Journal of the Association of Avian Veterinarians, V7, P79, DOI 10.2307/30135008

[8] MULTISYSTEMIC ADENOVIRUS INFECTION IN BROILER CHICKS WITH HYPOGLYCEMIA AND SPIKING MORTALITY [J].

GOODWIN, MA ;

HILL, DL ;

DEKICH, MA ;

PUTNAM, MR .

AVIAN DISEASES, 1993, 37 (02) :625-627

[9]

Grimes TM, 1992, P 41 W POULT DIS C S, P42

[10]

HSIUNG GD, 1982, DIAGNOSTIC VIROLOGY, P71

← 1 2 →