A method for direct DNA amplification of uncharacterized DNA viruses and for development of a viral polymerase chain reaction assay: Application to the red sea bream iridovirus

被引：22

作者：

Oshima, S

Hata, J

Segawa, C

Hirasawa, N

Yamashita, S

机构：

[1] NIPPON SUISAN KAISHA LTD,CENT RES LAB,HACHIOJI,TOKYO 192,JAPAN

[2] NIPPON SUISAN KAISHA LTD,OHITA MARINE CTR,TSURUMI,OITA 5088,JAPAN

来源：

ANALYTICAL BIOCHEMISTRY | 1996年 / 242卷 / 01期

关键词：

D O I：

10.1006/abio.1996.0421

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A method is described for isolating a DNA segment of a virus for which no protein or DNA sequence information is available, This segment can then be used to develop a PCR-based assay for the virus. The method is based on the widespread presence and strong conservation of the ribonucleotide reductase gene among DNA viruses. The validity of the procedure is demonstrated by development of an assay for the fish iridovirus, We report the direct isolation from infected fish of a 738-bp segment of the iridovirus ribonucleotide reductase small subunit gene without prior virus purification, Using the sequence information obtained, a PCR-based diagnostic system was developed for detecting iridovirus infection. (C) 1996 Academic Press, Inc.

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页码：15 / 19

页数：5

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