Mouse maelstrom, a component of nuage, is essential for spermatogenesis and transposon repression in meiosis

被引：270

作者：

Soper, Sarah F. C. ^{[2
]}

van der Heijden, Godfried W. ^{[1
]}

Hardiman, Tara C. ^{[1
]}

Goodheart, Mary ^{[3
,4
]}

Martin, Sandra L. ^{[5
,6
]}

de Boer, Peter ^{[7
]}

Bortvin, Alex ^{[1
]}

机构：

[1] Carnegie Inst Sci, Dept Embryol, Baltimore, MD 21218 USA

[2] Johns Hopkins Univ, Dept Biol, Baltimore, MD 21218 USA

[3] MIT, Howard Hughes Med Inst, Whitehead Inst, Cambridge, MA 02142 USA

[4] MIT, Dept Biol, Cambridge, MA 02142 USA

[5] Univ Colorado, Dept Cell & Dev Biol, Aurora, CO 80045 USA

[6] Univ Colorado, Program Mol Biol, Aurora, CO 80045 USA

[7] Radboud Univ Nijmegen, Med Ctr, Dept Obstet & Gynaecol, NL-6500 HB Nijmegen, Netherlands

来源：

DEVELOPMENTAL CELL | 2008年 / 15卷 / 02期

关键词：

D O I：

10.1016/j.devcel.2008.05.015

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

Tight control of transposon activity is essential for the integrity of the germline. Recently, a germ-cell-specific organelle, nuage, was proposed to play a role in transposon repression. To test this hypothesis, we disrupted a murine homolog of a Drosophila nuage protein Maelstrom. Effects on male meiotic chromosome synapsis and derepression of transposable elements (TEs) were observed. In the adult Mael(-/-) testes, LINE-1 (L1) derepression occurred at the onset of meiosis. As a result, Mael(-/-) spermatocytes were flooded with L1 ribonucleoproteins (RNPs) that accumulated in large cytoplasmic enclaves and nuclei. Mael(-/-) spermatocytes with nuclear L1 RNPs exhibited massive DNA damage and severe chromosome asynapsis even in the absence of SP011-generated meiotic double-strand breaks. This study demonstrates that MAEL, a nuage component, is indispensable for the silencing of TEs and identifies the initiation of meiosis as an important step in TE control in the male germline.

引用

页码：285 / 297

页数：13

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