LSD1 regulates the balance between self-renewal and differentiation in human embryonic stem cells

被引：251

作者：

Adamo, Antonio ^{[1
]}

Sese, Borja ^{[1
]}

Boue, Stephanie ^{[1
]}

Castano, Julio ^{[1
]}

Paramonov, Ida ^{[1
]}

Barrero, Maria J. ^{[1
]}

Izpisua Belmonte, Juan Carlos ^{[1
,2
]}

机构：

[1] Ctr Regenerat Med Barcelona, Barcelona 08003, Spain

[2] Salk Inst Biol Studies, La Jolla, CA 92037 USA

来源：

NATURE CELL BIOLOGY | 2011年 / 13卷 / 06期

关键词：

PROTEIN-INTERACTION NETWORK; DEVELOPMENTAL REGULATORS; TRANSCRIPTION; PROMOTES; GENES; RNA; DEMETHYLATION; METHYLATION; EXPRESSION; REPRESSION;

D O I：

10.1038/ncb2246

中图分类号：

Q2 [细胞生物学];

学科分类号：

071009 ; 090102 ;

摘要：

We identify LSD1 (lysine-specific demethylase 1; also known as KDM1A and A0F2) as a key histone modifier that participates in the maintenance of pluripotency through the regulation of bivalent domains, a chromatin environment present at the regulatory regions of developmental genes that contains both H3K4 di/trimethylation and H3K27 trimethylation marks. LSD1 occupies the promoters of a subset of developmental genes that contain bivalent domains and are co-occupied by OCT4 and NANOG in human embryonic stem cells, where it controls the levels of H3K4 methylation through its demethylase activity. Thus, LSD1 has a role in maintaining the silencing of several developmental genes in human embryonic stem cells by regulating the critical balance between H3K4 and H3K27 methylation at their regulatory regions.

引用

页码：652 / U265

页数：20

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