Bioconjugates for tunable peptide fragmentation: Free radical initiated peptide sequencing (FRIPS)

被引：126

作者：

Hodyss, R ^{[1
]}

Cox, HA ^{[1
]}

Beauchamp, JL ^{[1
]}

机构：

[1] CALTECH, Div Chem & Chem Engn, Pasadena, CA 91125 USA

来源：

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY | 2005年 / 127卷 / 36期

关键词：

D O I：

10.1021/ja052042z

中图分类号：

O6 [化学];

学科分类号：

0703 ;

摘要：

The free radical initiator Vazo 68 is coupled to a peptide and electrosprayed into an ion trap mass spectrometer. On collisional activation, the Vazo 68-peptide conjugate generates a free radical, which can be collisionally activated to cleave the peptide backbone. Mostly z-type fragments are formed, as in CAD of other radical peptides and ECD fragmentation. We present data for the Angiotensin II-Vazo 68 conjugate and discuss possible sites of H atom abstraction from the peptide. This experimental methodology for generating peptide fragments is a useful step toward the development of a completely gas-phase approach to protein sequencing. Copyright © 2005 American Chemical Society.

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页码：12436 / 12437

页数：2

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