Flagellar genes from Rhodobacter sphaeroides are homologous to genes of the fliF operon of Salmonella typhimurium and to the type-III secretion system

被引：7

作者：

Ballado, T

Campos, A

Camarena, L

Dreyfus, G

机构：

[1] UNIV NACL AUTONOMA MEXICO,INST FISIOL CELULAR,DEPT BIOENERGET,MEXICO CITY 04510,DF,MEXICO

[2] UNIV NACL AUTONOMA MEXICO,INST INVEST BIOMED,DEPT BIOL MOLEC,MEXICO CITY 04510,DF,MEXICO

来源：

GENE | 1996年 / 170卷 / 01期

关键词：

photosynthetic bacterium; cloning; sequencing; bacterial motility; export of virulence factors;

D O I：

10.1016/0378-1119(95)00855-1

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

A flagellar region of the genome of Rhodobacter sphaeroides was cloned and sequenced. Three ORFs were identified and arranged in the same order as fliH, fliI and fliJ of Salmonella typhimurium (St). ORF2 is highly similar to FliI from St (49% similarity) showing Walker's A and B motifs. Similar scores were found with proteins of the type-III secretion system of virulence factors. ORF3 shows 16.4 and 11.1% similarity to FliJ from St and Bacillus subtilis, respectively. This work also shows that ORF3 is similar to HrpJ5 from Pseudomonas syringae (19.2% similarity). It was found that ORF2 and ORF3 start immediately downstream from the adjacent coding region, suggesting a single transcriptional unit.

引用

页码：69 / 72

页数：4

共 18 条

[1] THE FLAA LOCUS OF BACILLUS-SUBTILIS IS PART OF A LARGE OPERON CODING FOR FLAGELLAR STRUCTURES, MOTILITY FUNCTIONS, AND AN ATPASE-LIKE POLYPEPTIDE [J].