High-throughput analysis of hexosamine using a colorimetric method

被引：9

作者：

Cheng, Yu-Shen ^{[1
]}

Labavitch, John ^{[2
]}

VanderGheynst, Jean S. ^{[1
]}

机构：

[1] Univ Calif Davis, Dept Biol & Agr Engn, Davis, CA 95616 USA

[2] Univ Calif Davis, Dept Plant Sci, Davis, CA 95616 USA

来源：

ANALYTICAL BIOCHEMISTRY | 2011年 / 408卷 / 01期

关键词：

CELL-WALL; GLUCOSAMINE; CHITOSAN; BIOMASS; LIGNIN;

D O I：

10.1016/j.ab.2010.08.022

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A 96-well plate method was developed for analysis of total hexosamine content in biological samples. Four hexosamine monomer derivatives glucosamine hydrochloride, glucosamine sulfate, galactosamine hydrochloride, and mannosamine hydrochloride were examined for the linearity of their spectra in the concentration range specified in the assay. The hexosamine concentration analysis range was linear from 0.1 to 1 mM. The quantification of hexosamines from chitin and chitosan upon acid hydrolysis was also tested. Accurate quantification of glucosamine content in chitin and chitosan with different molecular sizes and degrees of acetylation was demonstrated using the new method. (C) 2010 Elsevier Inc. All rights reserved.

引用

页码：160 / 162

页数：3

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