Metabolomic Approach with LC-QTOF to Study the Effect of a Nutraceutical Treatment on Urine of Diabetic Rats

被引：49

作者：

Godzien, Joanna ^{[1
,2
]}

Ciborowski, Michal ^{[1
,3
]}

Angulo, Santiago ^{[1
]}

Ruperez, Francisco J. ^{[1
]}

Paz Martinez, Ma ^{[1
]}

Senorans, Francisco J. ^{[4
]}

Cifuentes, Alejandro ^{[5
]}

Ibanez, Elena ^{[5
]}

Barbas, Coral ^{[1
]}

机构：

[1] San Pablo CEU Univ, Fac Pharm, CEMBIO, Madrid 28668, Spain

[2] John Paul II Catholic Univ Lublin, Dept Mol Biol, Fac Math & Nat Sci, PL-20718 Lublin, Poland

[3] Med Univ Bialystok, Dept Phys Chem, PL-15089 Bialystok, Poland

[4] Univ Autonoma Madrid, Secc Dept Ciencias Alimentac, E-28049 Madrid, Spain

[5] CSIC, Food Sci Res Inst, Dept Food Anal & Bioact, E-28049 Madrid, Spain

来源：

JOURNAL OF PROTEOME RESEARCH | 2011年 / 10卷 / 02期

关键词：

LC-MS; biostatistics; urine fingerprinting; antioxidants; Rosmarinus officinalis; folic acid; ROSEMARY ROSMARINUS-OFFICINALIS; SUPERCRITICAL-FLUID EXTRACTS; OXIDATIVE STRESS; HYPERGLYCEMIA; ANTIOXIDANTS; PLASMA; MS;

D O I：

10.1021/pr100993x

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The rat treated with streptozotocin has been proposed as the most appropriate model of systemic oxidative stress for studying antioxidant therapies. In that sense, rosemary extracts have long been recognized as having antioxidant properties, and folic acid may be able to improve endothelial progenitor cell function. A mixture containing both has been tested as a possible nutraceutical to improve health complications in diabetes. We have developed the methodology to evaluate metabolic changes in the urine of streptozotocin-induced diabetic rats after supplementing their diet with rosemary extract obtained with supercritical fluids (SFE) containing 10% folic acid in an acute but short-term study. It has been done with a metabolomics approach using LC-QTOF as an analytical tool. About 20 endogenous metabolites have been identified by databases and MS/MS showing statistically significant changes. Among them, several amino acids and their metabolites point to changes due to the effect of the gut microbiota. In addition, the comparison between control and streptozotocin-diabetic rats has permitted the showing of some metabolic coincidences between type 1 diabetes and other (possible) autoimmune diseases such as autism and/or Crohn's disease, and the nutraceutical intervention has succeeded in inducing changes in such biomarkers.

引用

页码：837 / 844

页数：8

共 39 条

[1] In vivo assessment of antidiabetic and antioxidant activities of rosemary (Rosmarinus officinalis) in alloxan-diabetic rabbits [J].