Peripheral tolerance in T cell receptor-transgenic mice: Evidence for T cell anergy

被引:50
作者
Falb, D
Briner, TJ
Sunshine, GH
Bourque, CR
Luqman, M
Gefter, ML
Kamradt, T
机构
[1] IMMULOG PHARMACEUT CORP,WALTHAM,MA
[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02139
[3] TUFTS UNIV,NEW ENGLAND MED CTR,SCH MED,DEPT MED,BOSTON,MA 02111
关键词
peripheral tolerance; T cell energy; transgenic mice;
D O I
10.1002/eji.1830260120
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
T cell tolerance can be induced in adult mice by injection of soluble antigenic peptide. The underlying mechanism has been difficult to establish in normal mice due to the low precursor frequency of T cells specific for any given antigen. Therefore, we examined peripheral tolerance in mice transgenic for a T cell receptor specific for a cytochrome c peptide bound to I-E(k). Antigen-specific hyporesponsiveness could be induced in the transgenic mice. We followed the transgene-bearing T cells with a clonotypic monoclonal antibody and found similar numbers of clonotypic T cells in tolerized and control mice. To prevent de novo differentiation of T cells we analyzed thymectomized mice in which antigen-specific hyporesponsiveness was induced. Our analysis of thymectomized transgenic mice showed that antigen-specific T cell hyporesponsiveness following injection of peptide intravenously is not caused by gross elimination of T cells. These data provide evidence for the role of anergy in peripheral tolerance.
引用
收藏
页码:130 / 135
页数:6
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