Proteomic and functional analysis of Argonaute-containing mRNA-protein complexes in human cells

被引：282

作者：

Hoeck, Julia

Weinmann, Lasse

Ender, Christine

Ruedel, Sabine

Kremmer, Elisabeth

Raabe, Monika

Urlaub, Henning

Meister, Gunter ^{[1
]}

机构：

[1] Max Planck Inst Biochem, Lab RNA Biol, Martinsried, Germany

[2] GSF Munich, Inst Mol Immunol, Munich, Germany

[3] Max Planck Inst Biophys Chem, Bioanalyt Mass Spectrometry Grp, Gottingen, Germany

来源：

EMBO REPORTS | 2007年 / 8卷 / 11期

关键词：

Argonaute proteins; gene silencing; microRNA; RNA interference;

D O I：

10.1038/sj.embor.7401088

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Members of the Argonaute ( Ago) protein family associate with small RNAs and have important roles in RNA silencing. Here, we analysed Ago1- and Ago2- containing protein complexes in human cells. Separation of Ago- associated messenger ribonucleoproteins ( mRNPs) showed that Ago1 and Ago2 reside in three complexes with distinct Dicer and RNA- induced silencing complex activities. A comprehensive proteomic analysis of Ago- containing mRNPs identified a large number of proteins involved in RNA metabolism. By using co- immunoprecipitation experiments followed by RNase treatment, we biochemically mapped interactions within Ago mRNPs. Using reporter assays and knockdown experiments, we showed that the putative RNA- binding protein RBM4 is required for microRNA- guided gene regulation.

引用

页码：1052 / 1060

页数：9

共 28 条

[1] MRNA degradation by miRNAs and GW182 requires both CCR4:NOT deadenylase and DCP1:DCP2 decapping complexes [J].