Simultaneous analysis of circulating human cytokines using a high-sensitivity cytokine biochip array

被引:30
作者
FitzGerald, S. Pete [1 ]
McConnell, R. Ivan [1 ]
Huxley, Allen [1 ]
机构
[1] Randox Labs, Crumlin BT29 4QY, Antrim, North Ireland
关键词
biochip; microarrays; cytokineso; detectability; multianalyte; multiplex; quantification; profiling; high sensitivity; immunoassays;
D O I
10.1021/pr070409o
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Biochip array technology allows the simultaneous measurement of multiple analytes per sample using a single analytical device. This study shows its applicability to the simultaneous measurement of 12 circulating human cytokines with high-sensitivity detection. This application increases their real-time detectability, maintaining a broad concentration range and without compromising the precision. This methodology represents a very applicable tool in cytokine research when simultaneous determination of minute concentrations can be of interest.
引用
收藏
页码:450 / 455
页数:6
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