Effect of heavy metal ion excess on sunflower leaves: Evidence for involvement of oxidative stress

被引:559
作者
Gallego, SM [1 ]
Benavides, MP [1 ]
Tomaro, ML [1 ]
机构
[1] UNIV BUENOS AIRES, FAC FARM & BIOQUIM, DEPT QUIM BIOL, RA-1113 BUENOS AIRES, DF, ARGENTINA
关键词
antioxidants; heavy metals; Helianthus annuus; oxidative stress;
D O I
10.1016/S0168-9452(96)04528-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The relationship between heavy metal ion toxicity and oxidative stress in plant cells was studied. Leaf segments from 14 day old sunflower seedlings were incubated in solutions containing 0.5 mM Fe(II), Cu(II) or Cd(II) ions for 12 h in the light. Treatment with metal ions studied produced a decrease in chlorophyll and GSH contents as well as increases in lipid peroxidation and lipoxygenase activity. Free radical scavengers, such as sodium benzoate and mannitol, prevented the decrease in chlorophyll and GSH content and the lipid peroxidation and lipoxygenase increases. While Fe(II) and Cd(II) ions caused a decrease in superoxide dismutase activity, Cu(II) ions raised its level. However, all three metal ions caused decreases in other antioxidant enzymes (catalase, ascorbate peroxidase, glutathione reductase and dehydroascorbate reductase). Free radical scavengers protected these enzymes against inactivation, No effect of these scavengers was observed on superoxide dismutase activity, These results indicate that excess Fe(II), Cu(II) or Cd(II) ions produce oxidative damage in plant leaves. Copyright (C) 1996 Elsevier Science Ireland Ltd
引用
收藏
页码:151 / 159
页数:9
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