Sex differences in the location of immunochemically defined cell populations in the mouse preoptic area/anterior hypothalamus

被引:50
作者
Wolfe, CA [1 ]
Van Doren, M [1 ]
Walker, HJ [1 ]
Seney, ML [1 ]
McClellan, KM [1 ]
Tobet, SA [1 ]
机构
[1] Colorado State Univ, Dept Biomed Sci, Ft Collins, CO 80523 USA
来源
DEVELOPMENTAL BRAIN RESEARCH | 2005年 / 157卷 / 01期
关键词
sexual differentiation; migration; cell death; cell fate; neurogenesis; gonadal steroids;
D O I
10.1016/j.devbrainres.2005.03.001
中图分类号
Q [生物科学];
学科分类号
07 ; 0710 ; 09 ;
摘要
The preoptic area/anterior hypothalamus (POA/AH) is sexually dimorphic in many vertebrates. We have defined specific cell populations within the POA/AH using immunocytochemical markers for estrogen receptor beta (ER beta) and the R1 subunit of the GABA(B) receptor (GABA(B)R1). Our previous finding of sex differences in cell migration in this region in embryonic day 15 mice led us to examine sex differences in the location or size of chemically identified cell groups. At embryonic day 17 (E17), cells containing immunoreactive (it) ER beta in females were located more dorsal and lateral than those in males. In contrast to this positional sex difference seen at E 17, ER expression at PO and adulthood showed a sex difference in cell number and area of immunoreactivity with a higher expression of ER beta in males than females. Furthermore, in animals that were genetically deprived of gonadal and adrenal hormones by virtue of a disrupted gene coding for steroidogenic factor 1, cells containing ir ER beta followed a female phenotype for location at E 17 and a female phenotype for number of ir cells at PO regardless of genetic sex, suggesting that circulating hormones may be influencing cell position in the POA/AH. A second phenotypically identified cell group containing ir GABA(B)R1 also had a sex difference in cell positions at E17. Females expressed GABA(B)R1 in cells with a more dorsal position than in males. These results provide support for the suggestion that sex differences in cellular organization in the developing hypothalamus arise from sex differences in cell migration. (c) 2005 Elsevier B.V. All rights reserved.
引用
收藏
页码:34 / 41
页数:8
相关论文
共 50 条
[1]  
ALLEN LS, 1989, J NEUROSCI, V9, P497
[2]  
[Anonymous], TOKUSHIMA J EXP MED
[3]   ANDROGEN ENHANCES NEURONAL DEGENERATION IN THE DEVELOPING PREOPTIC AREA - APOPTOSIS IN THE ANTEROVENTRAL PERIVENTRICULAR NUCLEUS (AVPVN-POA) [J].
ARAI, Y ;
MURAKAMI, S ;
NISHIZUKA, M .
HORMONES AND BEHAVIOR, 1994, 28 (04) :313-319
[4]   GABA receptor antagonists modulate postmitotic cell migration in slice cultures of embryonic rat cortex [J].
Behar, TN ;
Schaffner, AE ;
Scott, CA ;
Greene, CL ;
Barker, JL .
CEREBRAL CORTEX, 2000, 10 (09) :899-909
[5]   CYTOARCHITECTONIC SEXUAL DIMORPHISMS OF THE MEDIAL PREOPTIC AND ANTERIOR HYPOTHALAMIC AREAS IN GUINEA-PIG, RAT, HAMSTER, AND MOUSE [J].
BLEIER, R ;
BYNE, W ;
SIGGELKOW, I .
JOURNAL OF COMPARATIVE NEUROLOGY, 1982, 212 (02) :118-130
[6]   DISTRIBUTION OF GALANIN-IMMUNOREACTIVE CELLS WITHIN SEXUALLY DIMORPHIC COMPONENTS OF THE MEDIAL PREOPTIC AREA OF THE MALE AND FEMALE RAT [J].
BLOCH, GJ ;
ECKERSELL, C ;
MILLS, R .
BRAIN RESEARCH, 1993, 620 (02) :259-268
[7]   The preoptic area anterior hypothalamus of different strains of mice: sex differences and development [J].
Brown, AE ;
Mani, S ;
Tobet, SA .
DEVELOPMENTAL BRAIN RESEARCH, 1999, 115 (02) :171-182
[8]  
BYNE W, 1987, J NEUROSCI, V7, P2688
[9]   The interstitial nuclei of the human anterior hypothalamus: an investigation of sexual variation in volume and cell size, number and density [J].
Byne, W ;
Lasco, MS ;
Kemether, E ;
Shinwari, A ;
Edgar, MA ;
Morgello, S ;
Jones, LB ;
Tobet, S .
BRAIN RESEARCH, 2000, 856 (1-2) :254-258
[10]  
Chung WCJ, 2000, J NEUROBIOL, V43, P234, DOI 10.1002/(SICI)1097-4695(20000605)43:3<234::AID-NEU2>3.0.CO