Introduction of a novel pathway for IAA biosynthesis to rhizobia alters vetch root nodule development

被引：61

作者：

Camerini, Serena ^{[1
]}

Senatore, Beatrice ^{[1
]}

Lonardo, Enza ^{[1
]}

Imperlini, Esther ^{[1
]}

Bianco, Carmen ^{[1
]}

Moschetti, Giancarlo ^{[2
]}

Rotino, Giuseppe L. ^{[3
]}

Campion, Bruno ^{[3
]}

Defez, Roberto ^{[1
]}

机构：

[1] CNR, Inst Genet & Biophys, I-80131 Naples, Italy

[2] Univ Palermo, SENFIMIZO, Palermo, Italy

[3] Res Inst Vegetable Crops, CRA ISPORT, Montanaso Lombardo, LO, Italy

来源：

ARCHIVES OF MICROBIOLOGY | 2008年 / 190卷 / 01期

关键词：

IAA; ARA; auxin; nitrogen fixation; nodule zone; Vicia hirsuta;

D O I：

10.1007/s00203-008-0365-7

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

We introduced into Rhizobium leguminosarum bv. viciae LPR1105 a new pathway for the biosynthesis of the auxin, indole-3-acetic acid (IAA), under the control of a stationary phase-activated promoter active both in free-living bacteria and bacteroids. The newly introduced genes are the iaaM gene from Pseudomonas savastanoi and the tms2 gene from Agrobacterium tumefaciens. Free-living bacteria harbouring the promoter-iaaMtms2 construct release into the growth medium 14-fold more IAA than the wild-type parental strain. This IAA overproducing R. l. viciae, the RD20 strain, elicits the development of vetch root nodules containing up to 60-fold more IAA than nodules infected by the wild-type strain LPR1105. Vetch root nodules derived from RD20 are fewer in number per plant, heavier in terms of dry weight and show an enlarged and more active meristem. A significant increase in acetylene reduction activity was measured in nodules elicited in vetch by RD20.

引用

页码：67 / 77

页数：11

共 65 条

[1] RELATIONSHIP BETWEEN INDOLE-3-ACETIC-ACID LEVELS IN APPLE (MALUS-PUMILA MILL) ROOTSTOCKS CULTURED INVITRO AND ADVENTITIOUS ROOT-FORMATION IN THE PRESENCE OF INDOLE-3-BUTYRIC ACID [J].