Tiny microbes, enormous impacts: what matters in gut microbiome studies?

被引：118

作者：

Debelius, Justine ^{[1
]}

Song, Se Jin ^{[1
,2
]}

Vazquez-Baeza, Yoshiki ^{[3
]}

Xu, Zhenjiang Zech ^{[1
]}

Gonzalez, Antonio ^{[1
]}

Knight, Rob ^{[1
,3
]}

机构：

[1] Univ Calif San Diego, Dept Pediat, La Jolla, CA 92093 USA

[2] Univ Colorado, Dept Ecol & Evolutionary Biol, Boulder, CO 80309 USA

[3] Univ Calif San Diego, Dept Comp Sci & Engn, La Jolla, CA 92093 USA

来源：

GENOME BIOLOGY | 2016年 / 17卷

基金：

美国国家卫生研究院;

关键词：

PUMP INHIBITORS ALTER; BACTERIAL COMPOSITION; COLONIC MICROBIOME; FECAL SAMPLES; BODY HABITATS; METAANALYSES; DIVERSITY; DIET; METABOLISM; SEQUENCES;

D O I：

10.1186/s13059-016-1086-x

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Many factors affect the microbiomes of humans, mice, and other mammals, but substantial challenges remain in determining which of these factors are of practical importance. Considering the relative effect sizes of both biological and technical covariates can help improve study design and the quality of biological conclusions. Care must be taken to avoid technical bias that can lead to incorrect biological conclusions. The presentation of quantitative effect sizes in addition to P values will improve our ability to perform meta-analysis and to evaluate potentially relevant biological effects. A better consideration of effect size and statistical power will lead to more robust biological conclusions in microbiome studies.

引用

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