Kinetics of IL-6 and TNF-α changes in a canine model of sepsis induced by endotoxin

被引：62

作者：

Song, Ruhui ^{[1
]}

Kim, Junhwan ^{[1
]}

Yu, Dohyeon ^{[2
]}

Park, Chul ^{[1
]}

Park, Jinho ^{[1
]}

机构：

[1] Chonbuk Natl Univ, Dept Vet Internal Med, Coll Vet Med, Jeonju 561756, South Korea

[2] Univ Missouri, Coll Vet Med, Dept Vet Med & Surg, Columbia, MO 65211 USA

来源：

VETERINARY IMMUNOLOGY AND IMMUNOPATHOLOGY | 2012年 / 146卷 / 02期

基金：

新加坡国家研究基金会;

关键词：

Sepsis; IL-6; TNF-alpha; Gene expression; Experimental septic model; TUMOR-NECROSIS-FACTOR; INFLAMMATORY RESPONSE SYNDROME; CYTOKINES; CRITERIA; PLASMA;

D O I：

10.1016/j.vetimm.2012.02.008

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

071005 [微生物学]; 100108 [医学免疫学];

摘要：

Sepsis is a major cause of death in veterinary medicine, although a better prognosis can result from an early diagnosis. To speed the diagnosis, the biomarkers TNF-alpha and IL-6 can provide valuable information regarding systemic inflammatory response. The purpose of this study was to investigate the changes in cytokine levels in an experimental model of sepsis using ELISA and real-time PCR. Ten adult Beagles were studied; seven received an IV bolus of high dose lipopolysaccharide solution (1 mg/kg) to induce sepsis. The remaining three beagles were the control group. Blood samples were collected before and 1, 3, 6, 12, 24 and 48 h after administering LPS. Serum IL-6 level peaked at 3 h (1.89 +/- 0.10 ng/ml) and serum TNF-alpha peaked at 1 h (1.11 +/- 0.01 ng/ml). The expression of IL-6 mRNA in peripheral blood mononuclear cells (PBMC) increased 62-fold compared to the control group at 1 h; TNF-alpha mRNA increased by 4.5-fold at 1 h. The expressions of IL-6 and TNF-alpha mRNA in PBMCs changed more rapidly than serum IL-6 and TNF-alpha concentrations. In addition, TNF-alpha mRNA levels in PBMCs remained elevated longer than serum TNF-alpha. Our study establishes the basis for future work aimed at a better understanding of the systemic inflammatory response to infection and sepsis in canine patients. (C) 2012 Elsevier B.V. All rights reserved.

引用

页码：143 / 149

页数：7

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