Characterization of MarR superrepressor mutants

被引：33

作者：

Alekshun, MN

Levy, SB

机构：

[1] Tufts Univ, Sch Med, Ctr Adaptat Genet & Drug Resistance, Boston, MA 02111 USA

[2] Tufts Univ, Sch Med, Dept Mol Biol & Microbiol, Boston, MA 02111 USA

[3] Tufts Univ, Sch Med, Dept Med, Boston, MA 02111 USA

来源：

JOURNAL OF BACTERIOLOGY | 1999年 / 181卷 / 10期

关键词：

D O I：

10.1128/JB.181.10.3303-3306.1999

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

MarR negatively regulates expression of the multiple antibiotic resistance (mar) locus in Escherichia coli. Superrepressor mutants, generated in order to study regions of MarR required for function, exhibited altered inducer recognition properties in whole cells and increased DNA binding to marO in vitro. Mutations occurred in three areas of the relatively small MarR protein (144 amino acids). It is surmised that superrepression results from increased DNA binding activities of these mutant proteins.

引用

页码：3303 / 3306

页数：4

共 22 条

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