Synthesis and characterization of both ionically and enzymatically cross-linkable alginate

被引：175

作者：

Sakai, Shinji ^{[1
]}

Kawakami, Koel ^{[1
]}

机构：

[1] Kyushu Univ, Fac Engn, Dept Chem Engn, Nishi Ku, Fukuoka 8190395, Japan

来源：

ACTA BIOMATERIALIA | 2007年 / 3卷 / 04期

关键词：

alginate; peroxidase; enzyme-catalyzed gelation; oxidative coupling; immobilization;

D O I：

10.1016/j.actbio.2006.12.002

中图分类号：

R318 [生物医学工程];

学科分类号：

0831 [生物医学工程];

摘要：

Alginate with phenol moieties in the polymer side chains was synthesized through the conjugation reaction of alginate and tyramine. Immersing an aqueous solution of the alginate containing horseradish peroxidase into a solution containing H2O2 caused the solution to gel via peroxidase-catalyzed oxidative coupling of the phenols. In addition, alginate prepared under appropriate reaction conditions retained the attractive properties associated with unmodified alginate; spherical gel beads were formed by dropping an aqueous alginate solution (1.0 wt.%) into a solution containing calcium ions. The oxidative coupling of the phenols was effective for suppressing the destabilization of the alginate gel resulting from a loss of bonding between the divalent cations and alginate. The mechanical properties of the resultant gels were influenced by the preparation conditions of the alginate and the type of cross-linking. (c) 2006 Acta Materialia Inc. Published by Elsevier Ltd. All rights reserved.

引用

页码：495 / 501

页数：7

共 14 条

[1]

Improvement in the mucoadhesive properties of alginate by the covalent attachment of cysteine [J].