Limited signal transduction repertoire of human Y5 neuropeptide Y receptors expressed in HEC-1B cells

被引：15

作者：

Bischoff, A

Püttmann, K

Kötting, A

Moser, C

Buschauer, A

Michel, MC ^{[1
]}

机构：

[1] Univ Essen, Dept Med, D-45122 Essen, Germany

[2] Univ Regensburg, Inst Pharm, D-93041 Regensburg, Germany

来源：

PEPTIDES | 2001年 / 22卷 / 03期

关键词：

G-protein; adenylyl cyclase; phospholipase C; calcium; protein kinase C; mitogen-activated protein kinase;

D O I：

10.1016/S0196-9781(01)00346-1

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In HEC-1B cells transfected with human Y-5 neuropeptide Y (NPY) receptors (but not in non-transfected cells) NPY inhibited forskolin-stimulated cAMP accumulation in a pertussis toxin-sensitive manner (-log EC50 8.88 +/- 0.25). Elevations of intracellular Ca2+ were largely restricted to very high NPY concentrations and similar in transfected and nontransfected cells. NPY did not increase inositol phosphate accumulation and did not activate a variety of isoforms of protein kinase C or mitogen-activated protein kinases. We conclude that at least upon expression in HEC-1B cells the signal transduction of Y-5 NPY receptors is limited to inhibition of cAMP accumulation. (C) 2001 Elsevier Science Inc. All rights reserved.

引用

页码：387 / 394

页数：8

共 50 条

[1] Y1 RECEPTORS FOR NEUROPEPTIDE-Y ARE COUPLED TO MOBILIZATION OF INTRACELLULAR CALCIUM AND INHIBITION OF ADENYLATE-CYCLASE [J].