Areca nut extract inhibits the growth, attachment, and matrix protein synthesis of cultured human gingival fibroblasts

被引:53
作者
Chang, MC
Kuo, MYP
Hahn, LJ
Hsieh, CC
Lin, SK
Jeng, JH
机构
[1] Natl Taiwan Univ, Coll Med, Sch Dent, Lab Dent Pharmacol Toxicol, Taipei, Taiwan
[2] Chang Gung Inst Nursing, Team Biomed Sci, Tao Yuan, Taiwan
关键词
betel adverse effects; quid chewing; cell adhesion; cell division; fibroblasts; gingiva drug effects; periodontal diseases pathogenesis;
D O I
10.1902/jop.1998.69.10.1092
中图分类号
R78 [口腔科学];
学科分类号
1003 ;
摘要
BETEL QUID CHEWING IS a popular oral habit in India, South Africa, and many Southeast Asian countries. The effects of areca nut (AN) extract on the growth, attachment, and protein synthesis of healthy human gingival fibroblasts (GF) were investigated to determine why betel quid (BQ) chewers have higher prevalence of periodontal disease than non-chewers. Twenty-four hour exposure of human GF to AN extract (> 200 mu g/ml) in culture led to the formation of numerous intracellular vacuoles. As analyzed by modified MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl-tetrazolium bromide] assay, AN extract significantly suppressed the growth of GF over 5 days of incubation in a dose-dependent manner. At concentrations of 50 and 300 mu g/ml, AN extract suppressed the growth of GF with 30% and 57% (P < 0.05), respectively. AN extract also significantly suppressed the synthesis of [H-3]proline incorporation into trichloroacetic acid (TCA) precipitated proteins. At concentrations of 200, 400, and 600 mu g/ml, AN extract suppressed the protein synthesis with 33%, 58%, and 63% of inhibition (P < 0.05), respectively. Preincubation of cells in a medium containing AN extract for 2 hours inhibits the subsequent attachment of cultured GF to type I collagen at the 50% inhibitory concentration (IC50) which is about 720 to 798 mu g/ml. Considering the frequent consumption of BQ throughout the day, impairment of sequential fibroblast functions by BQ ingredients is a potential mechanism through which BQ chewing exert a deleterious effect to the gingival tissues.
引用
收藏
页码:1092 / 1097
页数:6
相关论文
共 26 条