A mouse model of intracerebral hemorrhage using autologous blood infusion

被引：130

作者：

Rynkowski, Michal A. ^{[1
]}

Kim, Grace H. ^{[1
]}

Komotar, Ricardo J. ^{[1
]}

Otten, Marc L. ^{[1
]}

Ducruet, Andrew F. ^{[1
]}

Zacharia, Brad E. ^{[1
]}

Kellner, Christopher P. ^{[1
]}

Hahn, David K. ^{[1
]}

Merkow, Maxwell B. ^{[1
]}

Garrett, Matthew C. ^{[1
]}

Starke, Robert M. ^{[1
]}

Cho, Byung-Moon ^{[1
]}

Sosunov, Sergei A. ^{[1
]}

Connolly, E. Sander ^{[1
]}

机构：

[1] Columbia Univ, Dept Neurol Surg, New York, NY 10032 USA

来源：

NATURE PROTOCOLS | 2008年 / 3卷 / 01期

关键词：

D O I：

10.1038/nprot.2007.513

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The development of controllable and reproducible animal models of intracerebral hemorrhage (ICH) is essential for the systematic study of the pathophysiology and treatment of hemorrhagic stroke. In recent years, we have used a modified version of a murine ICH model to inject blood into mouse basal ganglia. According to our protocol, autologous blood is stereotactically infused in two stages into the right striatum to mimic the natural events of hemorrhagic stroke. Following ICH induction, animals demonstrate reproducible hematomas, brain edema formation and marked neurological deficits. Our technique has proven to be a reliable and reproducible means of creating ICH in mice in a number of acute and chronic studies. We believe that our model will serve as an ideal paradigm for investigating the complex pathophysiology of hemorrhagic stroke. The protocol for establishing this model takes about 2 h.

引用

页码：122 / 128

页数：7

共 43 条

[1] Experimental animal models of intracerebral hemorrhage [J].

Andaluz, N ;

Zuccarello, M ;

Wagner, KR .

NEUROSURGERY CLINICS OF NORTH AMERICA, 2002, 13 (03) :385-+

[2] SPECTRUM OF PRIMARY INTRACEREBRAL HEMORRHAGE IN PERTH, WESTERN-AUSTRALIA, 1989-90 - INCIDENCE AND OUTCOME [J].

ANDERSON, CS ;

CHAKERA, TMH ;

STEWARTWYNNE, EG ;

JAMROZIK, KD .

JOURNAL OF NEUROLOGY NEUROSURGERY AND PSYCHIATRY, 1994, 57 (08) :936-940

[3] RAT MIDDLE CEREBRAL-ARTERY OCCLUSION - EVALUATION OF THE MODEL AND DEVELOPMENT OF A NEUROLOGIC EXAMINATION [J].

BEDERSON, JB ;

PITTS, LH ;

TSUJI, M ;

NISHIMURA, MC ;

DAVIS, RL ;

BARTKOWSKI, H .

STROKE, 1986, 17 (03) :472-476

[4] Experimental intracerebral hemorrhage in the mouse - Histological, behavioral, and hemodynamic characterization of a double-injection model [J].

Belayev, L ;

Saul, I ;

Curbelo, K ;

Busto, R ;

Belayev, A ;

Zhang, YB ;

Riyamongkol, P ;

Zhao, WZ ;

Ginsberg, MD .

STROKE, 2003, 34 (09) :2221-2227

[5] Sensorimotor and cognitive deficits after transient middle cerebral artery occlusion in the mouse [J].

Bouet, Valentine ;

Freret, Thomas ;

Toutain, Jerome ;

Divoux, Didier ;

Boulouard, Michel ;

Schumann-Bard, Pascale .

EXPERIMENTAL NEUROLOGY, 2007, 203 (02) :555-567

[6] Guidelines for the management of spontaneous intracerebral hemorrhage - A statement for healthcare professionals from a special writing group of the Stroke Council, American Heart Association [J].

Broderick, JP ;

Adams, HP ;

Barsan, W ;

Feinberg, W ;

Feldmann, E ;

Grotta, J ;

Kase, C ;

Krieger, D ;

Mayberg, M ;

Tilley, B ;

Zabramski, JM ;

Zuccarello, M .

STROKE, 1999, 30 (04) :905-915

[7] Use of a spectrophotometric hemoglobin assay to objectively quantify intracerebral hemorrhage in mice [J].

Choudhri, TF ;

Hoh, BL ;

Solomon, RA ;

Connolly, ES ;

Pinsky, DJ .

STROKE, 1997, 28 (11) :2296-2302

[8]

Clark WM, 1997, NEUROL RES, V19, P641

[9] Procedural and strain-related variables significantly affect outcome in a murine model of focal cerebral ischemia [J].

Connolly, ES ;

Winfree, CJ ;

Stern, DM ;

Solomon, RA ;

Pinsky, DJ .

NEUROSURGERY, 1996, 38 (03) :523-531

[10]

Deinsberger W, 1996, NEUROL RES, V18, P475

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