Sturgeon orphanin, a molecular "fossil" that bridges the gap between the opioids and orphanin FQ/nociceptin

被引:26
作者
Danielson, PB
Hoversten, MT
Fitzpatrick, M
Schreck, C
Akil, H
Dores, RM
机构
[1] Univ Denver, Dept Sci Biol, Denver, CO 80210 USA
[2] Univ Michigan, Mental Hlth Res Inst, Ann Arbor, MI 48109 USA
[3] Oregon State Univ, Dept Fisheries & Wildlife, Corvallis, OR 97331 USA
[4] Oregon State Univ, Oregon Cooperat Fish & Wildlife Res Unit, US Geol Survey, Biol Resources Div, Corvallis, OR 97331 USA
关键词
D O I
10.1074/jbc.M011741200
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
The elucidation of the cDNA sequence for sturgeon proorphanin provides a unique window for interpreting the evolutionary history of the opioid/orphanin gene family. The molecular "fossil" status of this precursor can be seen in several ancestral sequence characteristics that point to its origin as a duplication of either a prodynorphin- or proenkephalin-like gene. The sturgeon proorphanin cDNA encodes a precursor protein of 194 residues, and the orphanin heptadecapeptide itself binds not only the opioid receptor-like 1 (ORL1) receptor but also the classical (mu, kappa, and delta) opioid receptors with near equal affinity. Allowing for this broad receptor specificity are several amino acid substitutions at key positions in the heptadecapeptide sequence, relative to its mammalian orthologs, that have been linked by amino acid scans and site directed mutagenic studies to the exclusion of mammalian orphanin FQ/nociceptin from classic opioid ligands (i.e. F1Y and L14W). The unique receptor binding profile of sturgeon orphanin not only provides insight into the evolutionary history of the opioid and opioid-related peptides but also provides an ideal context in which to investigate the under lying mechanisms by which novel and often divergent physiological functions arise in receptor-ligand systems.
引用
收藏
页码:22114 / 22119
页数:6
相关论文
共 35 条
[1]   Endogenous opioids: overview and current issues [J].
Akil, H ;
Owens, C ;
Gutstein, H ;
Taylor, L ;
Curran, E ;
Watson, S .
DRUG AND ALCOHOL DEPENDENCE, 1998, 51 (1-2) :127-140
[2]   Gapped BLAST and PSI-BLAST: a new generation of protein database search programs [J].
Altschul, SF ;
Madden, TL ;
Schaffer, AA ;
Zhang, JH ;
Zhang, Z ;
Miller, W ;
Lipman, DJ .
NUCLEIC ACIDS RESEARCH, 1997, 25 (17) :3389-3402
[3]   HIGH-EFFICIENCY TRANSFORMATION OF MAMMALIAN-CELLS BY PLASMID DNA [J].
CHEN, C ;
OKAYAMA, H .
MOLECULAR AND CELLULAR BIOLOGY, 1987, 7 (08) :2745-2752
[4]  
CHENG Y, 1973, BIOCHEM PHARMACOL, V22, P3099
[5]   Molecular evolution of the opioid/orphanin gene family [J].
Danielson, PB ;
Dores, RM .
GENERAL AND COMPARATIVE ENDOCRINOLOGY, 1999, 113 (02) :169-186
[6]   Nociceptin (Orphanin FQ) abolishes gestational and ovarian sex steroid-induced antinociception and induces hyperalgesia [J].
DawsonBasoa, M ;
Gintzler, AR .
BRAIN RESEARCH, 1997, 750 (1-2) :48-52
[7]  
DELSAL G, 1989, BIOTECHNIQUES, V7, P514
[8]  
DOUGLASS J, 1984, ANNU REV BIOCHEM, V53, P665
[9]   RAPID PRODUCTION OF FULL-LENGTH CDNAS FROM RARE TRANSCRIPTS - AMPLIFICATION USING A SINGLE GENE-SPECIFIC OLIGONUCLEOTIDE PRIMER [J].
FROHMAN, MA ;
DUSH, MK ;
MARTIN, GR .
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1988, 85 (23) :8998-9002
[10]  
GOLDSTEIN A, 1989, MOL PHARMACOL, V36, P265