Hydroxylation of androstenedione by resting Rhodococcus sp cells in organic media

被引：18

作者：

Angelova, B

Fernandes, P

Cruz, A

Pinheiro, HM

Mutafov, S

Cabral, JMS

机构：

[1] Bulgarian Acad Sci, Stephan Angeloff Inst Microbiol, BU-1113 Sofia, Bulgaria

[2] Inst Super Tecn, Ctr Engn Biol & Quim, P-1049001 Lisbon, Portugal

[3] Univ Lusofona Humanidades & Tecnol, P-1749024 Lisbon, Portugal

来源：

ENZYME AND MICROBIAL TECHNOLOGY | 2005年 / 37卷 / 07期

关键词：

Rhodococcus; steroids; bis-(2-ethylhexyl) phthalate; microbial transformation;

D O I：

10.1016/j.enzmictec.2005.04.014

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The use of organic solvent-based media for microbial 9 alpha-hydroxylation of androstenedione (AD) by resting Rhodococcus sp. cells was evaluated. Product yields higher than 90% were observed in a two-phase phthalate-buffer transformation medium (3: 1, v/v) containing 1.0 g L-1 AD. The results suggest that in these conditions the reaction of Delta(1)-dehydrogenation is considerably inhibited. The successful hydroxylation reaction with a product yield exceeding 60% was found to take place also in a phthalate medium under simultaneous forced aeration and maintenance of a minimum water content of about 3% (v/v). (c) 2005 Elsevier Inc. All rights reserved.

引用

收藏

页码：718 / 722

页数：5

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