Synthesis of ascorbyloleate by immobilized Candida antarctica lipases

被引：47

作者：

Adamczak, M

Bornscheuer, UT

Bednarski, W

机构：

[1] Univ Warmia & Mazury, Chair Food Biotechnol, PL-10718 Olsztyn, Poland

[2] Ernst Moritz Arndt Univ Greifswald, Dept Tech Chem & Biotechnol, Inst Chem & Biochem, D-17489 Greifswald, Germany

来源：

PROCESS BIOCHEMISTRY | 2005年 / 40卷 / 10期

关键词：

ascorbyloleate; Candida antarctica; immobilization; lipase; water activity;

D O I：

10.1016/j.procbio.2005.01.016

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

The lipase-catalyzed synthesis of ascorbyloleate was studied using different preparations of lipases from Candida antarctica. Conversion to ascorbyloleate was influenced by the type of lipase, the carrier used for immobilization, water activity and organic solvent. The highest conversion (63%) was found using Chirazyme L-2 immobilized on carrier C2, in acetone, using methyloleate as acyl donor (4-fold excess), at water activity 0.11 and in the presence of molecular sieves. (c) 2005 Elsevier Ltd. All rights reserved.

引用

页码：3177 / 3180

页数：4

共 19 条

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[2]

ON THE IMPORTANCE OF THE SUPPORT MATERIAL FOR ENZYMATIC-SYNTHESIS IN ORGANIC MEDIA - SUPPORT EFFECTS AT CONTROLLED WATER ACTIVITY [J].