Enhancement of laccase activity in liquid cultures of the ligninolytic fungus Pleurotus ostreatus by cotton stalk extract

被引：67

作者：

Ardon, O ^{[1
]}

Kerem, Z ^{[1
]}

Hadar, Y ^{[1
]}

机构：

[1] HEBREW UNIV JERUSALEM,FAC AGR,DEPT PLANT PATHOL & MICROBIOL,IL-76100 REHOVOT,ISRAEL

来源：

JOURNAL OF BIOTECHNOLOGY | 1996年 / 51卷 / 03期

关键词：

laccase; lignin degradation; mushroom; Pleurotus; white rot fungi;

D O I：

10.1016/S0168-1656(96)01597-0

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Laccase is one of the enzymes suggested to play a role in fungal ligninolytic systems. Cotton stalks were found to be an excellent substrate for Pleurotus cultivation. In this work, the effects of cotton-stalk extract (CSE) were studied. It was found to stimulate both fungal growth in surface cultures and extracellular laccase activity in submerged cultures. Induction of laccase activity was dependent upon the concentration of the supplemented extract. The stimulation of laccase was observed as early as 3 h after amendment of CSE. Laccase activity staining after separation by gel electrophoresis revealed two major bands in both control and the CSE-induced cultures. Copyright (C) 1996 Elsevier Science B.V.

引用

页码：201 / 207

页数：7

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