The occurrence of anastomosis formation and nuclear exchange in intact arbuscular mycorrhizal networks

被引:114
作者
Giovannetti, M
Fortuna, P
Citernesi, AS
Morini, S
Nuti, MP
机构
[1] Univ Pisa, CNR, Ctr Studio Microbiol Suolo, Dipartimento & Biotecnol Agrarie, I-56124 Pisa, Italy
[2] Univ Pisa, Dipartimento Coltivazione & Difesa Specie Legnose, I-56124 Pisa, Italy
关键词
arbuscular mycorrhizas; Glomus mosseae; extraradical mycelium; anastomosis; nuclear exchange; mycorrhizal networks; wood wide web;
D O I
10.1046/j.0028-646x.2001.00216.x
中图分类号
Q94 [植物学];
学科分类号
071001 ;
摘要
The widespread occurrence of anastomoses and nuclear migration in intact extraradical arbuscular mycorrhizal (AAA) networks is reported here. Visualization and quantification of intact extramatrical hyphae spreading from colonized roots into the surrounding environment was obtained by using a two-dimensional experimental model system. After 7 d the length of extraradical mycelium in the AM symbiont Glomus mosseae ranged from 5169 mm in Thymus vulgaris to 7096 mm in Prunus cerasifera and 7471 mm in Allium porrum, corresponding to 10, 16 and 40 mm mm(-1) root length, respectively. In mycelium spreading from colonized roots of P. cerasifera and T. vulgaris, contacts leading to hyphal fusion were 64% and 78%, with 0.46 and 0.51 anastomoses mm(-1) of hypha, respectively. Histochemical localization of succinate dehydrogenase activity in hyphal bridges demonstrated protoplasmic continuity, while the detection of nuclei in the hyphal bridges confirmed the viability of anastomosed hyphae. The ability of AM extraradical mycelium to form anastomosis and to exchange nuclei suggests that, beyond the nutritional flow, an information flow might also be active in the network.
引用
收藏
页码:717 / 724
页数:8
相关论文
共 37 条
[1]   FORMATION OF EXTERNAL HYPHAE IN SOIL BY 4 SPECIES OF VESICULAR ARBUSCULAR MYCORRHIZAL FUNGI [J].
ABBOTT, LK ;
ROBSON, AD .
NEW PHYTOLOGIST, 1985, 99 (02) :245-255
[2]   Architecture and developmental dynamics of the external mycelium of the arbuscular mycorrhizal fungus Glomus intraradices grown under monoxenic conditions [J].
Bago, B ;
Azcón-Aguilar, C ;
Piché, Y .
MYCOLOGIA, 1998, 90 (01) :52-62
[3]   THE STRUCTURE AND FUNCTION OF THE VEGETATIVE MYCELIUM OF ECTOMYCORRHIZAL PLANTS .5. FORAGING BEHAVIOR AND TRANSLOCATION OF NUTRIENTS FROM EXPLOITED LITTER [J].
BENDING, GD ;
READ, DJ .
NEW PHYTOLOGIST, 1995, 130 (03) :401-409
[4]   THE STRUCTURE AND FUNCTION OF THE VEGETATIVE MYCELIUM OF ECTOMYCORRHIZAL PLANTS .6. ACTIVITIES OF NUTRIENT MOBILIZING ENZYMES IN BIRCH LITTER COLONIZED BY PAXILLUS-INVOLUTUS (FR) FR [J].
BENDING, GD ;
READ, DJ .
NEW PHYTOLOGIST, 1995, 130 (03) :411-417
[5]   HETEROKARYON SELF-INCOMPATIBILITY IN GIBBERELLA-FUJIKUROI (FUSARIUM-MONILIFORME) [J].
CORRELL, JC ;
KLITTICH, CJR ;
LESLIE, JF .
MYCOLOGICAL RESEARCH, 1989, 93 :21-27
[6]   SIZE, DISTRIBUTION AND BIOMASS OF GENETS IN POPULATIONS OF SUILLUS-BOVINUS (L, FR) ROUSSEL REVEALED BY SOMATIC INCOMPATIBILITY [J].
DAHLBERG, A ;
STENLID, J .
NEW PHYTOLOGIST, 1994, 128 (02) :225-234
[7]   THE STRUCTURE AND FUNCTION OF THE VEGETATIVE MYCELIUM OF ECTOMYCORRHIZAL PLANTS .1. TRANSLOCATION OF C-14-LABELED CARBON BETWEEN PLANTS INTERCONNECTED BY A COMMON MYCELIUM [J].
FINLAY, RD ;
READ, DJ .
NEW PHYTOLOGIST, 1986, 103 (01) :143-156
[8]  
Fortuna P, 1996, TREE PHYSIOL, V16, P757
[9]  
FRANCIS R, 1984, NATURE, V307, P53, DOI 10.1038/307053a0