Salting-out thin-layer chromatography of several myorelaxants

The chromatographic behavior of five myorelaxant drugs has been studied under the conditions used for salting-out thin-layer chromatography (SOTLC) on cellulose and alumina. For this purpose, aqueous ammonium sulfate solutions of different concentration were used as mobile phases. It was established that hR(F) values always decreased in parallel to increasing salt concentrations. When cellulose was used as adsorbent, a linear relationship was observed between the R-M values and the ammonium sulfate content of the mobile phase. Regression data of the plots obtained were used to determine the lipophilicity parameters R-M(0) and C-0. Lipophilicity determined in this way correlated with calculated log P values.