Full-genome RNAi profiling of early embryogenesis in Caenorhabditis elegans

被引:692
作者
Sönnichsen, B
Koski, LB
Walsh, A
Marschall, P
Neumann, B
Brehm, M
Alleaume, AM
Artelt, J
Bettencourt, P
Cassin, E
Hewitson, M
Holz, C
Khan, M
Lazik, S
Martin, C
Nitzsche, B
Ruer, M
Stamford, J
Winzi, M
Heinkel, R
Röder, M
Finell, J
Häntsch, H
Jones, SJM
Jones, M
Piano, F
Gunsalus, KC
Oegema, K
Gönczy, P
Coulson, A
Hyman, AA
Echeverri, CJ
机构
[1] Cenix Biosci GmbH, D-01307 Dresden, Germany
[2] Max Planck Inst Cell Biol & Genet, D-01307 Dresden, Germany
[3] British Columbia Canc Res Ctr, Genome Sci Ctr, Vancouver, BC V5Z 4E6, Canada
[4] Wellcome Trust Sanger Inst, Cambridge CB10 1SA, England
[5] NYU, Dept Biol, Ctr Comparat Funct Genom, New York, NY 10003 USA
基金
英国医学研究理事会;
关键词
D O I
10.1038/nature03353
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
A key challenge of functional genomics today is to generate well-annotated data sets that can be interpreted across different platforms and technologies. Large-scale functional genomics data often fail to connect to standard experimental approaches of gene characterization in individual laboratories. Furthermore, a lack of universal annotation standards for phenotypic data sets makes it difficult to compare different screening approaches. Here we address this problem in a screen designed to identify all genes required for the first two rounds of cell division in the Caenorhabditis elegans embryo. We used RNA-mediated interference to target 98% of all genes predicted in the C. elegans genome in combination with differential interference contrast time-lapse microscopy. Through systematic annotation of the resulting movies, we developed a phenotypic profiling system, which shows high correlation with cellular processes and biochemical pathways, thus enabling us to predict new functions for previously uncharacterized genes.
引用
收藏
页码:462 / 469
页数:8
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