The hESC line Envy expresses high levels of GFP in all differentiated progeny

被引:103
作者
Costa, M [1 ]
Dottori, M [1 ]
Ng, E [1 ]
Hawes, SM [1 ]
Sourris, K [1 ]
Jamshidi, P [1 ]
Pera, MF [1 ]
Elefanty, AG [1 ]
Stanley, EG [1 ]
机构
[1] Monash Immunol & Stem Cell Lab, Clayton, Vic 3800, Australia
关键词
D O I
10.1038/NMETH748
中图分类号
Q5 [生物化学];
学科分类号
071010 ; 081704 ;
摘要
Human embryonic stem cells (hESCs) have been advanced as a potential source of cells for use in cell replacement therapies. The ability to identify hESCs and their differentiated progeny readily in transplantation experiments will facilitate the analysis of hESC potential and function in vivo. We have generated a hESC Line designated 'Envy', in which robust levels of green fluorescent protein (GFP) are expressed in stem cells and all differentiated progeny.
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收藏
页码:259 / 260
页数:2
相关论文
共 9 条
[1]   Integration and differentiation of human embryonic stem cells transplanted to the chick embryo [J].
Goldstein, RS ;
Drukker, M ;
Reubinoff, BE ;
Benvenisty, N .
DEVELOPMENTAL DYNAMICS, 2002, 225 (01) :80-86
[2]   Stable genetic modification of human embryonic stem cells by lentiviral vectors [J].
Gropp, M ;
Itsykson, P ;
Singer, O ;
Ben-Hur, T ;
Reinhartz, E ;
Galun, E ;
Reubinoff, BE .
MOLECULAR THERAPY, 2003, 7 (02) :281-287
[3]   Maintenance of pluripotency in human embryonic stem cells stably over-expressing enhanced green fluorescent protein [J].
Liu, YP ;
Dovzhenko, OV ;
Garthwaite, MA ;
Dambaeva, SV ;
Durning, M ;
Pollastrini, LM ;
Golos, TG .
STEM CELLS AND DEVELOPMENT, 2004, 13 (06) :636-645
[4]   High-level sustained transgene expression in human embryonic stem cells using lentiviral vectors [J].
Ma, Y ;
Ramezani, A ;
Lewis, R ;
Hawley, RG ;
Thomson, JA .
STEM CELLS, 2003, 21 (01) :111-117
[5]   Activation of EGFP expression by Cre-mediated excision in a new ROSA26 reporter mouse strain [J].
Mao, XH ;
Fujiwara, Y ;
Chapdelaine, A ;
Yang, HD ;
Orkin, SH .
BLOOD, 2001, 97 (01) :324-326
[6]   Neural progenitors from human embryonic stem cells [J].
Reubinoff, BE ;
Itsykson, P ;
Turetsky, T ;
Pera, MF ;
Reinhartz, E ;
Itzik, A ;
Ben-Hur, T .
NATURE BIOTECHNOLOGY, 2001, 19 (12) :1134-1140
[7]   Embryonic stem cell lines from human blastocysts: somatic differentiation in vitro [J].
Reubinoff, BE ;
Pera, MF ;
Fong, CY ;
Trounson, A ;
Bongso, A .
NATURE BIOTECHNOLOGY, 2000, 18 (04) :399-404
[8]   Generalized lacZ expression with the ROSA26 Cre reporter strain [J].
Soriano, P .
NATURE GENETICS, 1999, 21 (01) :70-71
[9]   A novel cell-surface marker found on human embryonic hepatoblasts and a subpopulation of hepatic biliary epithelial cells [J].
Stamp, L ;
Crosby, HA ;
Hawes, SM ;
Strain, AJ ;
Pera, MF .
STEM CELLS, 2005, 23 (01) :103-112