Thin-layer chromatography separation of enantiomers of verapamil using macrocyclic antibiotic as a chiral selector

被引：30

作者：

Bhushan, R ^{[1
]}

Gupta, D ^{[1
]}

机构：

[1] Indian Inst Technol, Dept Chem, Roorkee 247667, Uttar Pradesh, India

来源：

BIOMEDICAL CHROMATOGRAPHY | 2005年 / 19卷 / 06期

关键词：

(+/-)-verapamil hydrochloride; macrocyclic antibiotics; vancomycin hydrochloride; TLC;

D O I：

10.1002/bmc.520

中图分类号：

Q5 [生物化学];

学科分类号：

071010 [生物化学与分子生物学]; 081704 [应用化学];

摘要：

Silica gel thin-layer chromatography plates impregnated with macrocyclic antibiotic, vancomycin, as chiral selector were prepared and used for the resolution of (+/-)-verapamil. A mobile phase system of acetonitrile-methanol-water (15:2.5:2.5, v/v) was worked out systematically. The effects of chiral selector, temperature and pH on resolution were also studied. The spots were detected with iodine vapors and the detection limit was found to be 0.074 mu g of each enantiomers. Copyright (C) 2005 John Wiley & Sons, Ltd.

引用

页码：474 / 478

页数：5

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