Accumulation of LOX-1 ligand in plasma and atherosclerotic lesions of Watanabe heritable hyperlipidemic rabbits: Identification by a novel enzyme immunoassay

被引:32
作者
Kakutani, M
Ueda, M
Naruko, T
Masaki, T
Sawamura, T [1 ]
机构
[1] Natl Cardiovasc Ctr, Res Inst, Dept Biosci, Osaka 5658565, Japan
[2] Osaka City Univ, Sch Med, Dept Pathol, Osaka 5458585, Japan
[3] Osaka City Gen Hosp, Dept Cardiol, Osaka 5340021, Japan
[4] Osaka Univ, Grad Sch Pharmaceut Sci, Dept Mol Pathophysiol, Suita, Osaka 5650871, Japan
关键词
lectin-like oxidized LDL receptor-1 (LOX-1); LOX-1; ligand; Watanabe heritable hyperlipidemic rabbit; atherosclerosis;
D O I
10.1006/bbrc.2001.4508
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
LOX-1 (lectin-like oxidized LDL receptor) is a newly identified cell surface receptor for oxidized LDL mainly expressed in endothelial cells. Recombinant soluble LOX-1(LOX-Fc) was generated by fusing the extracellular domain of LOX-1 with the Fc portion of IgG. A novel sandwich enzyme immunoassay specific for LOX-1 ligand is designed, using LOX-Fc and anti-apoB antibody. This immunoassay was used to determine LOX-1 ligand activity in normal and Watanabe heritable hyperlipidemic (WHHL) rabbit plasma. LOX-Fc was further applied for staining of atherosclerotic lesions of WHHL rabbits. LOX-I ligand levels were significantly elevated in the plasma of hyperlipidemic rabbits compared with controls. Furthermore, LOX-1 ligand activity was detected in the atherosclerotic lesions in situ. These results support the potential roles of LOX-1 interacting with its ligand in the pathogenesis of atherosclerosis, which is enhanced in hyperlipidemia. (C) 2001 Academic Press.
引用
收藏
页码:180 / 185
页数:6
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