Inhibition of malonaldehyde and acetaldehyde formation from blood plasma oxidation by naturally occurring antioxidants

The inhibitory effect of 2"-O-glycosylisovitexin (2"-O-GIV), 2,5-dimethyl-4-hydroxy-3(2H)-furanone (DMHF), L-ascorbic acid (vitamin C), and 4,4'-(isopropylidenedithio)bis(2,6-di-tert-butyl phenol) (probucol) on malonaldehyde (MA) and acetaldehyde formation from horse blood plasma oxidized with Fenton's reagent was determined by gas chromatography. 2"-O-GIV, DMHF, and L-ascorbic acid inhibited MA formation at a level of 100 nmol by 60, 22, and 22%, respectively; probucol did not show any inhibitory activity on MA formation. Improved inhibitory activity toward MA formation was observed when 2"-O-GIV or DMHF was mixed with L-ascorbic acid in equal amounts. Approximately 80-90% of acetaldehyde formation was inhibited by 300 nmol of 2"-O-GIV and probucol, whereas DMHF required 1000 nmol to exhibit the same level of inhibition.