A novel β-lactamase gene, bla(OXA-61) from Campylobacter jejuni GC015 was cloned and its nucleotide sequence determined. bla(OXA-61) encodes a protein of 257 amino acids in which the active-site STFK tetrad and conserved class D β-lactamase motifs YGN and KTG were identified. A conserved sequence upstream of bla(OXA-61) is required for expression in Campylobacter.