Interaction with heparin protects tissue transglutaminase against inactivation by heating and by proteolysis

被引：18

作者：

Gambetti, S

Dondi, A

Cervellati, C

Squerzanti, M

Pansini, FS

Bergamini, CM

机构：

[1] Univ Ferrara, Dept Biochem & Mol Biol, I-44100 Ferrara, Italy

[2] Univ Ferrara, ICSI, I-44100 Ferrara, Italy

[3] Univ Ferrara, Menopause & Osteoporosis Ctr, I-44100 Ferrara, Italy

来源：

BIOCHIMIE | 2005年 / 87卷 / 06期

关键词：

transglutaminase; heparin; thermal inactivation; microcalorimetry; limited proteolysis;

D O I：

10.1016/j.biochi.2005.01.012

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The considerable affinity of tissue transglutaminase for heparin was the basis for use of heparin-based affinity matrices for enzyme purification. Interaction of transglutaminase with heparin might mimic the physiological binding to membrane heparan sulfates, accounting for the limited but significant fraction of enzyme exposed at cell surface to crosslink ECM proteins. Exploring effects of heparin on transglutaminase activity and stability, we have noted that heparin only slightly affects activity in vitro, but the protein against heat treatment and proteolysis. (c) 2005 Elsevier SAS. All rights reserved.

引用

收藏

页码：551 / 555

页数：5

相关论文

共 28 条

[1] Cell-surface-associated tissue transglutaminase is a target of MMP-2 proteolysis [J].

Belkin, AM ;

Zemskov, EA ;

Hang, J ;

Akimov, SS ;

Sikora, S ;

Strongin, AY .

BIOCHEMISTRY, 2004, 43 (37) :11760-11769

[2] GTP MODULATES CALCIUM-BINDING AND CATION-INDUCED CONFORMATIONAL-CHANGES IN ERYTHROCYTE TRANSGLUTAMINASE [J].

BERGAMINI, CM .

FEBS LETTERS, 1988, 239 (02) :255-258

[3] Conformational stability of human erythrocyte transglutaminase - Patterns of thermal unfolding at acid and alkaline pH [J].

Bergamini, CM ;

Dean, M ;

Matteucci, G ;

Hanau, S ;

Tanfani, F ;

Ferrari, C ;

Boggian, M ;

Scatturin, A .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 266 (02) :575-582

[4] Role of transglutaminase 2 in glucose tolerance: knockout mice studies and a putative mutation in a MODY patient [J].

Bernassola, F ;

Federici, M ;

Corazzari, M ;

Terrinoni, A ;

Hribal, ML ;

De Laurenzi, V ;

Ranalli, M ;

Massa, O ;

Sesti, G ;

Mclean, WHI ;

Citro, G ;

Barbetti, F ;

Melino, G .

FASEB JOURNAL, 2002, 16 (11) :1371-1378

[5] Functions of cell surface heparan sulfate proteoglycans [J].

Bernfield, M ;

Götte, M ;

Park, PW ;

Reizes, O ;

Fitzgerald, ML ;

Lincecum, J ;

Zako, M .

ANNUAL REVIEW OF BIOCHEMISTRY, 1999, 68 :729-777

[6]

BORTH W, 1991, J BIOL CHEM, V266, P18149

[7] The structural basis for the regulation of tissue transglutaminase by calcium ions [J].

Casadio, R ;

Polverini, E ;

Mariani, P ;

Spinozzi, F ;

Carsughi, F ;

Fontana, A ;

de Laureto, PP ;

Matteucci, G ;

Bergamini, CM .

EUROPEAN JOURNAL OF BIOCHEMISTRY, 1999, 262 (03) :672-679

[8]

CHANG SK, 1986, J BIOL CHEM, V261, P8112

[9] Management of thrombotic and cardiovascular disorders in the new millenium [J].

Fareed, J ;

Hoppensteadt, DA ;

Bick, RL .

CLINICAL AND APPLIED THROMBOSIS-HEMOSTASIS, 2003, 9 (02) :101-108

[10] Transglutaminase induction by various cell death and apoptosis pathways [J].

Fesus, L ;

Madi, A ;

Balajthy, Z ;

Nemes, Z ;

Szondy, Z .

EXPERIENTIA, 1996, 52 (10-11) :942-949