ISWI is an ATP-dependent nucleosome remodeling factor

被引：200

作者：

Corona, DFV

Längst, G

Clapier, CR

Bonte, EJ

Ferrari, S

Tamkun, JW

Becker, PB ^{[1
]}

机构：

[1] European Mol Biol Lab, Gene Express Programme, D-69117 Heidelberg, Germany

[2] Univ Calif Santa Cruz, Dept Biol, Santa Cruz, CA 95064 USA

来源：

MOLECULAR CELL | 1999年 / 3卷 / 02期

关键词：

D O I：

10.1016/S1097-2765(00)80314-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The ATPase ISWI is a subunit of several distinct nucleosome remodeling complexes that increase the accessibility of DNA in chromatin. We found that the isolated ISWI protein itself was able to carry out nucleosome remodeling, nucleosome rearrangement, and chromatin assembly reactions. The ATPase activity of ISWI was stimulated by nucleosomes but not by free DNA or free histones, indicating that ISWI recognizes a specific structural feature of nucleosomes. Nucleosome remodeling, therefore, does not require a functional interaction between ISWI and the other subunits of ISWI complexes. The role of proteins associated with ISWI may be to regulate the activity of the remodeling engine or to define the physiological context within which a nucleosome remodeling reaction occurs.

引用

页码：239 / 245

页数：7

共 48 条

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