Genomic bacterial artificial chromosome library of the Japanese flounder Paralichthys olivaceus

被引：56

作者：

Katagiri, T

Asakawa, S

Hirono, I

Aoki, T

Shimizu, N

机构：

[1] Tokyo Univ Fisheries, Lab Genet & Biochem, Minato Ku, Tokyo 1088477, Japan

[2] Keio Univ, Sch Med, Dept Mol Biol, Shinjuku Ku, Tokyo 1608582, Japan

来源：

MARINE BIOTECHNOLOGY | 2000年 / 2卷 / 06期

关键词：

Japanese flounder; Paralichthys olivaceus; bacterial artificial chromosome (BAC); high-density replica filter;

D O I：

10.1007/s101260000041

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We have constructed a genomic bacterial artificial chromosome (BAC) library from homozygous cloned Japanese flounder Paralichthys olivaceus using the pBAC-lac vector. This BAC library consists of about 49,100 clones and is deposited in 128 microtiter plates with 384 wens. The average size of inserted DNA was calculated to be 165 kb. The BAC library was determined to cover 9 times the Japanese founder haploid genome. The Japanese flounder genomic EPIC library will be useful for gene isolation as well as quantitative trait loci (QTL) analysis.

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收藏

页码：571 / 576

页数：6

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