Fragmented population structure of Plasmodium falciparum in a region of declining endemicity

被引:66
作者
Anthony, TG
Conway, DJ
Cox-Singh, J
Matusop, A
Ratnam, S
Shamsul, S
Singh, B
机构
[1] Univ London London Sch Hyg & Trop Med, Dept Infect & Trop Med, London WC1E 7HT, England
[2] Univ Malaysia Sarawak, Fac Med & Hlth Sci, Kota Samarahan, Malaysia
[3] Sarawak State Dept Hlth, Kuching, Malaysia
[4] Sabah State Dept Hlth, Kota Kinabalu, Madagascar
基金
英国惠康基金;
关键词
D O I
10.1086/429338
中图分类号
R392 [医学免疫学]; Q939.91 [免疫学];
学科分类号
100102 ;
摘要
Background. The population genetic structure of Plasmodium falciparum differs between endemic regions, but the characteristics of a population recently fragmented by effective malaria control have been unknown. Methods. Genotypic analysis of 10 microsatellite loci widely separated in the parasite genome was conducted on 288 P. falciparum isolates from 8 foci in Malaysian Borneo, a region in which malaria incidence has been progressively reduced. Results. Within all P. falciparum foci, moderate levels of allelic diversity were found, but levels of multilocus linkage disequilibrium were extremely variable. The population with the highest proportion of mixed-clone infections also had the highest allelic diversity and nonsignificant linkage disequilibrium. In contrast, several populations showed evidence of clonal expansion, and one offshore island population had exceptionally high levels of linkage disequilibrium. Genetic differentiation between many populations was very high and strongly associated with the geographical distance between them. Conclusions. High levels of differentiation and contrasting population structure among P. falciparum populations in Malaysian Borneo indicate that they are genetically independent. This supports the feasibility of individually eradicating the remaining P. falciparum foci.
引用
收藏
页码:1558 / 1564
页数:7
相关论文
共 24 条
  • [1] Microsatellite markers reveal a spectrum of population structures in the malaria parasite Plasmodium falciparum
    Anderson, TJC
    Haubold, B
    Williams, JT
    Estrada-Franco, JG
    Richardson, L
    Mollinedo, R
    Bockarie, M
    Mokili, J
    Mharakurwa, S
    French, N
    Whitworth, J
    Velez, ID
    Brockman, AH
    Nosten, F
    Ferreira, MU
    Day, KP
    [J]. MOLECULAR BIOLOGY AND EVOLUTION, 2000, 17 (10) : 1467 - 1482
  • [2] Twelve microsatellite markers for characterization of Plasmodium falciparum from finger-prick blood samples
    Anderson, TJC
    Su, XZ
    Bockarie, M
    Lagog, M
    Day, KP
    [J]. PARASITOLOGY, 1999, 119 : 113 - 125
  • [3] Extreme geographical fixation of variation in the Plasmodium falciparum gamete surface protein gene Pfs48/45 compared with microsatellite loci
    Conway, DJ
    Machado, RLD
    Singh, B
    Dessert, P
    Mikes, ZS
    Povoa, MM
    Oduola, AMJ
    Roper, C
    [J]. MOLECULAR AND BIOCHEMICAL PARASITOLOGY, 2001, 115 (02) : 145 - 156
  • [4] High recombination rate in natural populations of Plasmodium falciparum
    Conway, DJ
    Roper, C
    Oduola, AMJ
    Arnot, DE
    Kremsner, PG
    Grobusch, MP
    Curtis, CF
    Greenwood, BM
    [J]. PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA, 1999, 96 (08) : 4506 - 4511
  • [5] Cornuet JM, 1996, GENETICS, V144, P2001
  • [6] Increased sensitivity of malaria detection by nested polymerase chain reaction using simple sampling and DNA extraction
    Cox-Singh, J
    Mahayet, S
    Abdullah, MS
    Singh, B
    [J]. INTERNATIONAL JOURNAL FOR PARASITOLOGY, 1997, 27 (12) : 1575 - 1577
  • [7] Cox-Singh Janet, 2001, American Journal of Tropical Medicine and Hygiene, V64, P28
  • [8] Significant linkage disequilibrium and high genetic diversity in a population of Plasmodium falciparum from an area (Republic of the Congo) highly endemic for malaria
    Durand, P
    Michalakis, Y
    Cestier, S
    Oury, B
    Leclerc, MC
    Tibayrenc, M
    Renaud, F
    [J]. AMERICAN JOURNAL OF TROPICAL MEDICINE AND HYGIENE, 2003, 68 (03) : 345 - 349
  • [9] Microsatellite characterization of plasmodium falciparum from cerebral and uncomplicated malaria patients in Southern Vietnam
    Ferreira, MU
    Nair, S
    Hyunh, TV
    Kawamoto, F
    Anderson, TJC
    [J]. JOURNAL OF CLINICAL MICROBIOLOGY, 2002, 40 (05) : 1854 - 1857
  • [10] FSTAT (Version 1.2): A computer program to calculate F-statistics
    Goudet, J
    [J]. JOURNAL OF HEREDITY, 1995, 86 (06) : 485 - 486