Total chemical synthesis of the 3′ untranslated region of the hepatitis C virus with long oligodeoxynucleotides

被引：6

作者：

Encke, J ^{[1
]}

Putlitz, JZ ^{[1
]}

Heintges, T ^{[1
]}

Wands, JR ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Massachusetts Gen Hosp, Ctr Canc,Mol Hepatol Lab, Charlestown, MA 02129 USA

来源：

JOURNAL OF VIROLOGICAL METHODS | 1998年 / 74卷 / 01期

关键词：

hepatitis C virus; oligodeoxynucleotides; chemical synthesis;

D O I：

10.1016/S0166-0934(98)00077-9

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Hepatitis C Virus (HCV) is the major causative agent of chronic hepatitis. Since it has been difficult to obtain full-length cDNA clones of HCV including the 3' untranslated region (UTR) that give rise to replication competent virus, we generated the 3'UTR by a modified protocol of total chemical synthesis (TCS) with overlap-extension-PCR using four long oligodeoxynucleotides. A synthetic cDNA fragment of about 340 nucleotides (nt) in length was generated, subcloned and sequenced. This approach represents a rapid and easy alternative to RT-PCR from infectious serum and may be a highly valuable method to generate partial cDNA clones of HCV and other viruses including defined variants. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：117 / 121

页数：5

共 16 条

[1] THE NATURAL-HISTORY OF COMMUNITY-ACQUIRED HEPATITIS-C IN THE UNITED-STATES [J].