Cy3B™:: Improving the performance of cyanine dyes

被引：104

作者：

Cooper, M ^{[1
]}

Ebner, A

Briggs, M

Burrows, M

Gardner, N

Richardson, R

West, R

机构：

[1] Amersham Biosci, Discovery Syst, Maynard Ctr, Cardiff CF14 7YT, S Glam, Wales

[2] Johannes Kepler Univ, Inst Biophys, A-4040 Linz, Austria

来源：

JOURNAL OF FLUORESCENCE | 2004年 / 14卷 / 02期

关键词：

CyDye; Cy3B; bioconjugation; fluorescence;

D O I：

10.1023/B:JOFL.0000016286.62641.59

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The spectral properties of a rigidified trimethine cyanine dye, Cy3B(TM) have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an antibody. This study compared the conjugated emission properties of Cy3B with other commercially available fluorophores emitting at similar wavelengths.

引用

页码：145 / 150

页数：6

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