Transformation of a Texas cotton cultivar by using Agrobacterium and the shoot apex

被引：78

作者：

Zapata, C ^{[1
]}

Park, SH ^{[1
]}

El-Zik, KM ^{[1
]}

Smith, RH ^{[1
]}

机构：

[1] Texas A&M Univ, Dept Soil & Crop Sci, College Stn, TX 77843 USA

来源：

THEORETICAL AND APPLIED GENETICS | 1999年 / 98卷 / 02期

关键词：

Gossypium hirsutum; transformation; kanamycin;

D O I：

10.1007/s001220051065

中图分类号：

S3 [农学（农艺学）];

学科分类号：

0901 ;

摘要：

Transgenic cotton (Gossypium hirsutum L.) plants of a Texas cultivar CUBQHRPIS were obtained using Agrobacterium-mediated transformation coupled with the use of shoot-apex explants. After inoculation with A. tumefaciens strain LBA 4404 containing the pBI121 plasmid, regeneration of primary plants was carried out in a medium containing kanamycin (100 mg l(-1)). Progeny obtained by selfing were germinated in the greenhouse and selected for expression of the T-DNA marker gene encoding neomycin phosphotransferase II (NPTII) by painting kanamycin (2%) on the leaves. Plants that survived the leaf painting were analyzed by DNA blots. Evidence for integration of the transgene (GLIS) was observed in two successive generations from the regenerants (T-0). The transformed plants appeared to have more than one copy of the T-DNA.

引用

页码：252 / 256

页数：5

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