Characterization of rat porin isoforms: cloning of a cardiac type-3 variant encoding an additional methionine at its putative N-terminal region

被引：24

作者：

Anflous, K

Blondel, O

Bernard, A

Khrestchatisky, M

Ventura-Clapier, R ^{[1
]}

机构：

[1] Univ Paris Sud, Fac Pharm, INSERM,U446, Lab Cardiol Cellulaire & Mol, F-92296 Chatenay Malabry, France

[2] Univ Paris 05, INSERM, U29, F-75014 Paris, France

来源：

BIOCHIMICA ET BIOPHYSICA ACTA-GENE STRUCTURE AND EXPRESSION | 1998年 / 1399卷 / 01期

关键词：

mitochondrion; permeability; ADP; striated muscle; voltage-dependent anion channel; porin; isoform; cloning; distribution;

D O I：

10.1016/S0167-4781(98)00088-8

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In vivo, the outer mitochondrial membrane presents a restriction of diffusion for ADP in heart and slow twitch skeletal muscles, but not in fast twitch skeletal muscle. Mitochondrial porins constitute the main pathway for the transit of metabolites across the outer mitochondrial membrane. We decided, therefore, to characterize, by cloning, rat heart VDAC and to follow their expression in different striated muscles. We cloned three isoforms, one being HVDAC1-like porin (RVDAC1) whereas the other two are MVDAC3-like porins (RVDAC3 and RVDAC3v). These three isoforms are ubiquitously expressed among striated muscles. RVDAC3v differs from RVDAC3 by one additional amino acid, a Met, located between Val(39) and Glu(40) in RVDAC3 sequence. This study constitutes a first step in order to further characterize striated muscle porin isoforms. (C) 1998 Elsevier Science B.V. All rights reserved.

引用

页码：47 / 50

页数：4

共 15 条

[1] STUDIES ON HUMAN PORIN .6. PRODUCTION AND CHARACTERIZATION OF 8 MONOCLONAL MOUSE ANTIBODIES AGAINST THE HUMAN VDAC PORIN 31HL AND THEIR APPLICATION FOR HISTOTOPOLOGICAL STUDIES IN HUMAN SKELETAL-MUSCLE [J].