RNA interference: A mammalian SID-1 homologue enhances siRNA uptake and gene silencing efficacy in human cells

被引：114

作者：

Duxbury, MS ^{[1
]}

Ashley, SW ^{[1
]}

Whang, EE ^{[1
]}

机构：

[1] Harvard Univ, Sch Med, Brigham & Womens Hosp, Dept Surg, Boston, MA 02115 USA

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 2005年 / 331卷 / 02期

关键词：

pancreatic; adenocarcinoma; cancer; RNA interference; RNAi; small interfering RNA; siRNA; efficiency; FLJ20174; SID-1;

D O I：

10.1016/j.bbrc.2005.03.199

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

SID-1 is a transmembrane protein that mediates systemic RNA interference in Caenorhabditis elegans. Here we show that the mammalian SID-1 homologue FLJ20174 localizes to the cell membrane of human cells and enhances their uptake of small interfering RNA (siRNA), resulting in increased siRNA-mediated gene silencing efficacy. This is the first demonstration to show that overexpression of a membrane protein enhances siRNA internalization in mammalian cells. This observation raises the possibility of enhancing the efficacy of RNA interference. (c) 2005 Elsevier Inc. All rights reserved.

引用

页码：459 / 463

页数：5

共 6 条

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