The cannabinoid receptor agonist WIN 55,212-2 reduces the initial cerebral damage after hypoxic-ischemic injury in fetal lambs

被引：26

作者：

Alonso-Alconada, Daniel ^{[1
]}

Alvarez, Francisco J. ^{[2
]}

Alvarez, Antonia ^{[1
]}

Mielgo, Victoria E. ^{[2
]}

Goni-de-Cerio, Felipe ^{[1
]}

Rey-Santano, Maria C. ^{[2
]}

Caballero, Amale ^{[3
]}

Martinez-Orgado, Jose ^{[4
]}

Hilario, Enrique ^{[1
]}

机构：

[1] Univ Basque Country, Dept Cell Biol & Histol, Sch Med, Leioa, Vizcaya, Spain

[2] Hosp Cruces, Unit Perinatal Res, Baracaldo, Vizcaya, Spain

[3] Univ Basque Country, Dept Neurosci, Sch Med, Leioa, Vizcaya, Spain

[4] Hosp Puerta Hierro, Madrid, Spain

来源：

BRAIN RESEARCH | 2010年 / 1362卷

关键词：

Hypoxia-ischemia; Brain damage; Apoptosis; Cannabinoid; FIBRILLARY ACIDIC PROTEIN; DELAYED NEURONAL DEATH; PROGRAMMED CELL-DEATH; CB2; RECEPTOR; BRAIN-DAMAGE; THERAPEUTIC STRATEGIES; MORPHOLOGICAL-CHANGES; MATTER ASTROCYTES; RAT MODEL; PRETERM;

D O I：

10.1016/j.brainres.2010.09.050

中图分类号：

Q189 [神经科学];

学科分类号：

071006 ;

摘要：

The aim of the present work was to evaluate in an early time point the effect of the cannabinoid agonist WIN 55,212-2 after hypoxic ischemic (HI) brain injury induced by partial occlusion of the umbilical cord of premature fetal lambs. Lambs were assigned to three experimental groups: one SHAM group: non-injured animals, and two hypoxic ischemic groups that received a dose of 0.01 mu g/kg WIN 55,212-2 (HI + WIN group) or not (HI + VEH) after 60 min of a hypoxic ischemic event. All animals were managed on mechanical ventilation for 3 h and then sacrificed. Brains were perfusion-fixed and different regions separated for regional cerebral blood flow measurement, apoptosis quantification by TUNEL method and S-100 protein analysis by flow cytometry. The number of apoptotic cells was lower in the HI + WIN group in all regions studied. Moreover, animals treated with the cannabinoid agonist showed higher values in the percentage of S-100 positive cells in all regions, except,in the cortex. In both studies we obtained similar values between SHAM group and HI + WIN group. Our results suggest that the administration of the cannabinoid agonist WIN 55,212-2 after hypoxic ischemic brain injury in preterm lambs decreases brain injury reducing the delayed cell death and glial damage. (C) 2010 Elsevier B.V. All rights reserved.

引用

页码：150 / 159

页数：10

共 61 条

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