Plant calcium-dependent protein kinase-related kinases (CRKs) do not require calcium for their activities

被引：50

作者：

Furumoto, T ^{[1
]}

Ogawa, N ^{[1
]}

Hata, S ^{[1
]}

Izui, K ^{[1
]}

机构：

[1] KYOTO UNIV,FAC AGR,APPL BOT LAB,SAKYO KU,KYOTO 60601,JAPAN

来源：

FEBS LETTERS | 1996年 / 396卷 / 2-3期

关键词：

calcium-dependent protein kinase; CDPK-related protein kinase; bacterial expression; cDNA cloning; maize;

D O I：

10.1016/0014-5793(96)01090-3

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In plants, calcium-dependent protein kinases (CDPKs) make up a large family that is characterized by a C-terminal calmodulin(CaM)-like domain. Recently, a novel carrot cDNA clone encoding an atypical CDPK, which has a significantly degenerate sequence in the CaM-like domain, was found and named CDPK-related protein kinase (CRK) [Lindzen, E. and Choi, J.H. (1995) Plant Mol. Biol. 28, 785-797]. We obtained two different cDNA clones from maize which encode CRKs. For the first enzymatic characterization of CRK, a maize cDNA clone was expressed in E. coli. The recombinant protein efficiently phosphorylated casein, a conventional protein substrate. Notably, in this in vitro phosphorylation assay, the kinase activity did not require calcium as an activator. Thus, CRKs were suggested to be novel calcium-independent protein kinases having a degenerate CaM domain, the function of which remains to be elucidated.

引用

页码：147 / 151

页数：5

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