Structure and Mechanism of the CMR Complex for CRISPR-Mediated Antiviral Immunity

被引：238

作者：

Zhang, Jing ^{[3
]}

Rouillon, Christophe ^{[3
]}

Kerou, Melina ^{[3
]}

Reeks, Judith ^{[3
]}

Brugger, Kim ^{[4
]}

Graham, Shirley ^{[3
]}

Reimann, Julia ^{[5
]}

Cannone, Giuseppe ^{[1
,2
]}

Liu, Huanting ^{[3
]}

Albers, Sonja-Verena ^{[5
]}

Naismith, James H. ^{[3
]}

Spagnolo, Laura ^{[1
,2
]}

White, Malcolm F. ^{[3
]}

机构：

[1] Univ Edinburgh, Inst Struct Mol Biol, Edinburgh EH9 3JR, Midlothian, Scotland

[2] Univ Edinburgh, Ctr Sci Extreme Condit, Edinburgh EH9 3JR, Midlothian, Scotland

[3] Univ St Andrews, St Andrews KY16 9ST, Fife, Scotland

[4] Univ Cambridge, Addenbrookes Hosp, EASIH, Cambridge CB2 0QQ, England

[5] Max Planck Inst Terr Microbiol, Archaeal Mol Biol Grp, D-35043 Marburg, Germany

来源：

MOLECULAR CELL | 2012年 / 45卷 / 03期

基金：

英国生物技术与生命科学研究理事会; 英国惠康基金;

关键词：

STREPTOCOCCUS-THERMOPHILUS; HYPERTHERMOPHILIC ARCHAEON; SULFOLOBUS-SOLFATARICUS; DYNAMIC PROPERTIES; CAS SYSTEMS; RNA; DEFENSE; INTERFERENCE; RECOGNITION; EVOLUTION;

D O I：

10.1016/j.molcel.2011.12.013

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The prokaryotic clusters of regularly interspaced palindromic repeats (CRISPR) system utilizes genomically encoded CRISPR RNA (crRNA), derived from invading viruses and incorporated into ribonucleoprotein complexes with CRISPR-associated (CAS) proteins, to target and degrade viral DNA or RNA on subsequent infection. RNA is targeted by the CMR complex. In Sulfolobus solfataricus, this complex is composed of seven CAS protein subunits (Cmr1-7) and carries a diverse "payload" of targeting crRNA. The crystal structure of Cmr7 and low-resolution structure of the complex are presented. S. solfataricus CMR cleaves RNA targets in an endo-nucleolytic reaction at UA dinucleotides. This activity is dependent on the 8 nt repeat-derived 5' sequence in the crRNA, but not on the presence of a proto-spacer-associated motif (PAM) in the target. Both target and guide RNAs can be cleaved, although a single molecule of guide RNA can support the degradation of multiple targets.

引用

页码：303 / 313

页数：11

共 43 条

[1] Production of recombinant and tagged proteins in the hyperthermophilic archaeon Sulfolobus solfataricus [J].

Albers, SV ;

Jonuscheit, M ;

Dinkelaker, S ;

Urich, T ;

Kletzin, A ;

Tampé, R ;

Driessen, AJM ;

Schleper, C .

APPLIED AND ENVIRONMENTAL MICROBIOLOGY, 2006, 72 (01) :102-111

[2] The Piwi-piRNA pathway provides an adaptive defense in the transposon arms race [J].

Aravin, Alexei A. ;

Hannon, Gregory J. ;

Brennecke, Julius .

SCIENCE, 2007, 318 (5851) :761-764

[3] Structure and activity of the Cas3 HD nuclease MJ0384, an effector enzyme of the CRISPR interference [J].

Beloglazova, Natalia ;

Petit, Pierre ;

Flick, Robert ;

Brown, Greg ;

Savchenko, Alexei ;

Yakunin, Alexander F. .

EMBO JOURNAL, 2011, 30 (22) :4616-4627

[4] Clustered regularly interspaced short palindrome repeats (CRISPRs) have spacers of extrachromosomal origin [J].

Bolotin, A ;

Ouinquis, B ;

Sorokin, A ;

Ehrlich, SD .

MICROBIOLOGY-SGM, 2005, 151 :2551-2561

[5] Small CRISPR RNAs guide antiviral defense in prokaryotes [J].

Brouns, Stan J. J. ;

Jore, Matthijs M. ;

Lundgren, Magnus ;

Westra, Edze R. ;

Slijkhuis, Rik J. H. ;

Snijders, Ambrosius P. L. ;

Dickman, Mark J. ;

Makarova, Kira S. ;

Koonin, Eugene V. ;

van der Oost, John .

SCIENCE, 2008, 321 (5891) :960-964

[6] Cas6 is an endoribonuclease that generates guide RNAs for invader defense in prokaryotes [J].

Carte, Jason ;

Wang, Ruiying ;

Li, Hong ;

Terns, Rebecca M. ;

Terns, Michael P. .

GENES & DEVELOPMENT, 2008, 22 (24) :3489-3496

[7] CRISPR RNA maturation by trans-encoded small RNA and host factor RNase III [J].

Deltcheva, Elitza ;

Chylinski, Krzysztof ;

Sharma, Cynthia M. ;

Gonzales, Karine ;

Chao, Yanjie ;

Pirzada, Zaid A. ;

Eckert, Maria R. ;

Vogel, Joerg ;

Charpentier, Emmanuelle .

NATURE, 2011, 471 (7340) :602-+

[8] Phage response to CRISPR-Encoded resistance in Streptococcus thermophilus [J].

Deveau, Helene ;

Barrangou, Rodolphe ;

Garneau, Josiane E. ;

Labonte, Jessica ;

Fremaux, Christophe ;

Boyaval, Patrick ;

Romero, Dennis A. ;

Horvath, Philippe ;

Moineau, Sylvain .

JOURNAL OF BACTERIOLOGY, 2008, 190 (04) :1390-1400

[9] CRISPR-based immune systems of the Sulfolobales: complexity and diversity [J].

Garrett, Roger A. ;

Shah, Shiraz A. ;

Vestergaard, Gisle ;

Deng, Ling ;

Gudbergsdottir, Ley ;

Kenchappa, Chandra S. ;

Erdmann, Susanne ;

She, Qunxin .

BIOCHEMICAL SOCIETY TRANSACTIONS, 2011, 39 :51-57

[10] Visualizing density maps with UCSF Chimera [J].

Goddard, Thomas D. ;

Huang, Conrad C. ;

Ferrin, Thomas E. .

JOURNAL OF STRUCTURAL BIOLOGY, 2007, 157 (01) :281-287

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