Studies of Fe3O4-chitosan nanoparticles prepared by co-precipitation under the magnetic field for lipase immobilization

被引:127
作者
Liu, Yong [1 ]
Jia, Shaoyi [1 ]
Wu, Qian [1 ]
Ran, Jingyu [1 ]
Zhang, Wei [1 ]
Wu, Songhai [1 ]
机构
[1] Tianjin Univ, Sch Chem Engn & Technol, Tianjin 300072, Peoples R China
关键词
Lipase; Fe3O4-chitosan nanoparticles; Co-precipitation; Magnetic field; Immobilization; IRON-OXIDE NANOPARTICLES; CANDIDA-RUGOSA LIPASE; BIOMEDICAL APPLICATIONS; PERFORMANCE; BIODIESEL; GROWTH; OIL;
D O I
10.1016/j.catcom.2010.12.032
中图分类号
O64 [物理化学(理论化学)、化学物理学];
学科分类号
070304 ; 081704 ;
摘要
A simple method was introduced to prepare magnetic Fe3O4-chitosan nanoparticles by co-precipitation with 0.45 T static magnetic field via glutaraldehyde cross-linking reaction, and the nanoparticles were used to immobilize lipase. The influence of magnetic field on the properties of magnetic nanoparticles was studied by SEM, XRD and VSM. The results showed that no obvious difference of the nanoparticles structure was found, while the morphology varied from random sphere-like to rod-like and magnetic character was significantly changed. Furthermore, the lipase immobilized with nanoparticles prepared under the magnetic field enhanced the activity, operational and storage stability compared with the control sample. Crown Copyright (c) 2010 Published by Elsevier B.V. All rights reserved.
引用
收藏
页码:717 / 720
页数:4
相关论文
共 22 条
[1]   Studies of optimum conditions for covalent immobilization of Candida rugosa lipase on poly(γ-glutamic acid) by RSM [J].
Chang, S. -W. ;
Shaw, J. -F. ;
Yang, K. -H. ;
Chang, S. -F. ;
Shieh, C. -J. .
BIORESOURCE TECHNOLOGY, 2008, 99 (08) :2800-2805
[2]   Characterization of aqueous dispersions of Fe3O4 nanoparticles and their biomedical applications [J].
Cheng, FY ;
Su, CH ;
Yang, YS ;
Yeh, CS ;
Tsai, CY ;
Wu, CL ;
Wu, MT ;
Shieh, DB .
BIOMATERIALS, 2005, 26 (07) :729-738
[3]   Lipase-mediated chiral resolution of racemates in organic solvents [J].
Ghanem, A ;
Aboul-Enein, HY .
TETRAHEDRON-ASYMMETRY, 2004, 15 (21) :3331-3351
[4]   Polymer synthesis by in vitro enzyme catalysis [J].
Gross, RA ;
Kumar, A ;
Kalra, B .
CHEMICAL REVIEWS, 2001, 101 (07) :2097-2124
[5]  
GUI YL, 2008, INT J BIOL MACROMOL, V42, P405
[6]   Synthesis and surface engineering of iron oxide nanoparticles for biomedical applications [J].
Gupta, AK ;
Gupta, M .
BIOMATERIALS, 2005, 26 (18) :3995-4021
[7]   Bacterial lipases: an overview of production, purification and biochemical properties [J].
Gupta, R ;
Gupta, N ;
Rathi, P .
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY, 2004, 64 (06) :763-781
[8]   Iron oxide nanoparticles-chitosan composite based glucose biosensor [J].
Kaushika, Ajeet ;
Khan, Raju ;
Solanki, Pratima R. ;
Pandey, Pratibha ;
Alam, Javed ;
Ahmad, Sharif ;
Malhotra, B. D. .
BIOSENSORS & BIOELECTRONICS, 2008, 24 (04) :676-683
[9]   Immobilized Candida antarctica lipase-catalyzed alcoholysis of cotton seed oil in a solvent-free medium [J].
Köse, Ö ;
Tüter, M ;
Aksoy, HA .
BIORESOURCE TECHNOLOGY, 2002, 83 (02) :125-129
[10]   Immobilized Pseudomonas cepacia lipase for biodiesel fuel production from soybean oil [J].
Noureddini, H ;
Gao, X ;
Philkana, RS .
BIORESOURCE TECHNOLOGY, 2005, 96 (07) :769-777