Quantitative isolation of microbial DNA from different types of soils of natural and agricultural ecosystems

被引：33

作者：

Blagodatskaya, EV ^{[1
]}

Blagodatskii, SA

Anderson, TH

机构：

[1] Russian Acad Sci, Inst Physicochim & Biol Problems Soil Sci, Pushchino 142290, Moscow Oblast, Russia

[2] FAL Inst Agroecol, Braunschweig, Germany

来源：

MICROBIOLOGY | 2003年 / 72卷 / 06期

基金：

俄罗斯基础研究基金会;

关键词：

soil microorganisms; DNA isolation; microbial biomass;

D O I：

10.1023/B:MICI.0000008379.63620.7b

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

A novel procedure was developed for direct quantitative isolation of microbial DNA from soil. This technique was used to evaluate microbial DNA pools in soils of contrasting types (chernozems and brown forest soils) under different anthropogenic loads. A strong correlation was found between microbial biomass and DNA contents in soils of different types (R-2 = 0.799). The ratio of soil CO2 emission rate to the amount of extractable DNA in the soil was shown to reflect the physiological state of the soil microbial community; this ratio can be used as an ecophysiological parameter similarly to the metabolic quotient qCO(2).

引用

页码：744 / 749

页数：6

共 19 条

[1]

Anderson J.P.E., 1978, SOIL BIOL BIOCHEM, V20, P107

[2] QUANTIFICATION OF BACTERIAL AND FUNGAL CONTRIBUTIONS TO SOIL RESPIRATION [J].