Micronucleus formation in V79 cells treated with respirable silica dispersed in medium and in simulated pulmonary surfactant

被引：28

作者：

Liu, XR ^{[1
]}

Keane, MJ ^{[1
]}

Zhong, BZ ^{[1
]}

Ong, TM ^{[1
]}

Wallace, WE ^{[1
]}

机构：

[1] NIOSH,HLTH EFFECTS RES LAB,MORGANTOWN,WV 26506

来源：

MUTATION RESEARCH-ENVIRONMENTAL MUTAGENESIS AND RELATED SUBJECTS | 1996年 / 361卷 / 2-3期

关键词：

silicon dioxide; quartz; V79 lung fibroblast; micronucleus induction; pulmonary surfactant; dipalmitoylphosphatidylcholine;

D O I：

10.1016/S0165-1161(96)90243-5

中图分类号：

X [环境科学、安全科学];

学科分类号：

08 ; 0830 ;

摘要：

Chinese hamster lung fibroblasts (V79 cells) were challenged with respirable silica particles using an in vitro genotoxicity assay. Two particle sizes of crystalline quartz and a non-crystalline silica were assayed for induction of micronuclei (MN) in V79 cells. Some of the silica dusts used were pretreated with simulated pulmonary surfactant to model in vivo exposure conditions. The results showed that both crystalline and non-crystalline silica dispersed in medium (MEM) induced MN formation in a dose-dependent manner. Crystalline silica was more active in this assay than non-crystalline silica on a mass basis. The results also show that the frequency of micronucleated cells in cultures treated with surfactant-coated silica was not significantly different from that of the non-treated control cultures. These results seem to indicate that silica can cause chromosomal aberrations and/or aneuploidies in V79 cells; however, pretreatment of silica particles with simulated pulmonary surfactant reduces or delays genotoxicity in this assay.

引用

页码：89 / 94

页数：6

共 15 条

[1] EFFECT OF SHORT-TERM EXOGENOUS PULMONARY SURFACTANT TREATMENT ON ACUTE LUNG DAMAGE ASSOCIATED WITH THE INTRATRACHEAL INSTILLATION OF SILICA [J].